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- W3164784445 abstract "Human APJ receptor/apelin receptor (APJR), activated by apelin peptide isoforms, regulates a wide range of physiological processes. The role of extracellular loop (ECL) domain residues of APJR in ligand binding and receptor activation has not been established yet. Based on multiple sequence alignment of APJ receptor from various organisms, we identified conserved residues in the extracellular domains. Alanine substitutions of specific residues were characterized to evaluate their ligand binding efficiency and G q ‐, G i ‐, and β‐arrestin‐mediated signaling. Mutation‐dependent variation in ligand binding and signaling was observed. W 197 A in ECL2 and L 276 L 277 W 279 ‐AAA in ECL3 were deficient in G i and β‐arrestin signaling pathways with relatively preserved G q ‐mediated signaling. T 169 T 170 ‐AA, Y 182 A, and T 190 A mutants in ECL2 showed impaired β‐arrestin‐dependent cell signaling while maintaining G protein ‐ mediated signaling. Structural comparison with angiotensin II type I receptor revealed the importance of ECL2 and ECL3 residues in APJR ligand binding and signaling. Our results unequivocally confirm the specific role of these ECL residues in ligand binding and in orchestrating receptor conformations that are involved in preferential/biased signaling functions." @default.
- W3164784445 created "2021-06-07" @default.
- W3164784445 creator A5002841195 @default.
- W3164784445 creator A5018492562 @default.
- W3164784445 creator A5028405824 @default.
- W3164784445 creator A5044233528 @default.
- W3164784445 date "2021-06-24" @default.
- W3164784445 modified "2023-09-25" @default.
- W3164784445 title "Critical APJ receptor residues in extracellular domains that influence effector selectivity" @default.
- W3164784445 cites W138314167 @default.
- W3164784445 cites W1491752347 @default.
- W3164784445 cites W1511152517 @default.
- W3164784445 cites W1584308350 @default.
- W3164784445 cites W1908561802 @default.
- W3164784445 cites W1960000138 @default.
- W3164784445 cites W1966741491 @default.
- W3164784445 cites W1967293793 @default.
- W3164784445 cites W1973342839 @default.
- W3164784445 cites W1975740938 @default.
- W3164784445 cites W1982193571 @default.
- W3164784445 cites W1986607505 @default.
- W3164784445 cites W1989706089 @default.
- W3164784445 cites W1989969172 @default.
- W3164784445 cites W2000993281 @default.
- W3164784445 cites W2008078163 @default.
- W3164784445 cites W2012515727 @default.
- W3164784445 cites W2013824152 @default.
- W3164784445 cites W2014447414 @default.
- W3164784445 cites W2020735591 @default.
- W3164784445 cites W2020905941 @default.
- W3164784445 cites W2022260308 @default.
- W3164784445 cites W2041375669 @default.
- W3164784445 cites W2045109604 @default.
- W3164784445 cites W2049611742 @default.
- W3164784445 cites W2052091241 @default.
- W3164784445 cites W2052328463 @default.
- W3164784445 cites W2053766719 @default.
- W3164784445 cites W2056779812 @default.
- W3164784445 cites W2059174703 @default.
- W3164784445 cites W2060660919 @default.
- W3164784445 cites W2061800712 @default.
- W3164784445 cites W2067551740 @default.
- W3164784445 cites W2089713519 @default.
- W3164784445 cites W2090092141 @default.
- W3164784445 cites W2101143553 @default.
- W3164784445 cites W2102513649 @default.
- W3164784445 cites W2104477830 @default.
- W3164784445 cites W2108886007 @default.
- W3164784445 cites W2109058223 @default.
- W3164784445 cites W2113521438 @default.
- W3164784445 cites W2119199305 @default.
- W3164784445 cites W2122844011 @default.
- W3164784445 cites W2127739127 @default.
- W3164784445 cites W2127774996 @default.
- W3164784445 cites W2131918497 @default.
- W3164784445 cites W2132939152 @default.
- W3164784445 cites W2137092708 @default.
- W3164784445 cites W2139934040 @default.
- W3164784445 cites W2144799280 @default.
- W3164784445 cites W2144998676 @default.
- W3164784445 cites W2149963584 @default.
- W3164784445 cites W2152256079 @default.
- W3164784445 cites W2152479803 @default.
- W3164784445 cites W2155885188 @default.
- W3164784445 cites W2158985985 @default.
- W3164784445 cites W2163865644 @default.
- W3164784445 cites W2165312739 @default.
- W3164784445 cites W2167857702 @default.
- W3164784445 cites W2296782701 @default.
- W3164784445 cites W2346738973 @default.
- W3164784445 cites W2406715792 @default.
- W3164784445 cites W2476813941 @default.
- W3164784445 cites W2517768455 @default.
- W3164784445 cites W2521352320 @default.
- W3164784445 cites W2557947909 @default.
- W3164784445 cites W2578882363 @default.
- W3164784445 cites W2615284011 @default.
- W3164784445 cites W2753077764 @default.
- W3164784445 cites W2754464795 @default.
- W3164784445 cites W275975138 @default.
- W3164784445 cites W2772835320 @default.
- W3164784445 cites W2784655325 @default.
- W3164784445 cites W2786245560 @default.
- W3164784445 cites W2887619083 @default.
- W3164784445 cites W2897059447 @default.
- W3164784445 cites W2905214631 @default.
- W3164784445 cites W2909017711 @default.
- W3164784445 cites W2918046511 @default.
- W3164784445 cites W2944411542 @default.
- W3164784445 cites W2999524723 @default.
- W3164784445 cites W3134069115 @default.
- W3164784445 doi "https://doi.org/10.1111/febs.16048" @default.
- W3164784445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34076959" @default.
- W3164784445 hasPublicationYear "2021" @default.
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