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• W3164861384 abstract "The crystallization and structural determination of large RNAs and their complexes remain major bottlenecks in the mechanistic analysis of cellular and viral RNAs. Here, we describe a protocol that combines postcrystallization dehydration and ion replacement that dramatically improved the diffraction quality of crystals of a large gene-regulatory tRNA–mRNA complex. Through this method, the resolution limit of X-ray data extended from 8.5 to 3.2 Å, enabling structure determination. Although this protocol was developed for a particular RNA complex, the general importance of solvent and counterions in nucleic acid structure may render it generally useful for crystallographic analysis of other RNAs." @default.
• W3164861384 created "2021-06-07" @default.
• W3164861384 creator A5046781760 @default.
• W3164861384 creator A5047073489 @default.
• W3164861384 date "2021-01-01" @default.
• W3164861384 modified "2023-09-26" @default.
• W3164861384 title "Improving RNA Crystal Diffraction Quality by Postcrystallization Treatment" @default.
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• W3164861384 doi "https://doi.org/10.1007/978-1-0716-1499-0_3" @default.
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