FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3165292893> ?p ?o ?g. }

• W3165292893 abstract "Abstract Three-dimensional models are considered a powerful tool for improving the concordance between in vitro and in vivo phenotypes. However, the duration of spheroid culture may influence the degree of correlation between these counterparts. When using immortalised cell lines as model systems, the assumption for consistency and reproducibility is often made without adequate characterization or validation. It is therefore essential to define the biology of each spheroid model by investigating proteomic dynamics, which may be altered relative to culture duration. As an example, we assessed the influence of culture duration on the relative proteome abundance of HepG2 cells cultured as spheroids, which are routinely used to model aspects of the liver. Quantitative proteomic profiling of whole cell lysates labelled with tandem-mass tags was conducted using liquid chromatography-tandem mass spectrometry (LC–MS/MS). In excess of 4800 proteins were confidently identified, which were shared across three consecutive time points over 28 days. The HepG2 spheroid proteome was divergent from the monolayer proteome after 14 days in culture and continued to change over the successive culture time points. Proteins representing the recognised core hepatic proteome, cell junction, extracellular matrix, and cell adhesion proteins were found to be continually modulated." @default.
• W3165292893 created "2021-06-07" @default.
• W3165292893 creator A5033320946 @default.
• W3165292893 creator A5043638361 @default.
• W3165292893 creator A5056103070 @default.
• W3165292893 creator A5058105609 @default.
• W3165292893 creator A5067604655 @default.
• W3165292893 date "2021-05-25" @default.
• W3165292893 modified "2023-09-26" @default.
• W3165292893 title "Continual proteomic divergence of HepG2 cells as a consequence of long-term spheroid culture" @default.
• W3165292893 cites W1789161693 @default.
• W3165292893 cites W1819665821 @default.
• W3165292893 cites W1879322546 @default.
• W3165292893 cites W1978542424 @default.
• W3165292893 cites W1982635432 @default.
• W3165292893 cites W1993557008 @default.
• W3165292893 cites W2010061535 @default.
• W3165292893 cites W2013620034 @default.
• W3165292893 cites W2019162141 @default.
• W3165292893 cites W2024229755 @default.
• W3165292893 cites W2025831725 @default.
• W3165292893 cites W2028921444 @default.
• W3165292893 cites W2036200411 @default.
• W3165292893 cites W2038747312 @default.
• W3165292893 cites W2047242615 @default.
• W3165292893 cites W2074563837 @default.
• W3165292893 cites W2084919734 @default.
• W3165292893 cites W2086694648 @default.
• W3165292893 cites W2109850567 @default.
• W3165292893 cites W2115109074 @default.
• W3165292893 cites W2120057036 @default.
• W3165292893 cites W2122183733 @default.
• W3165292893 cites W2135059893 @default.
• W3165292893 cites W2147830145 @default.
• W3165292893 cites W2162169885 @default.
• W3165292893 cites W2165122639 @default.
• W3165292893 cites W2272852695 @default.
• W3165292893 cites W2275160084 @default.
• W3165292893 cites W2321561488 @default.
• W3165292893 cites W2332155194 @default.
• W3165292893 cites W2345415005 @default.
• W3165292893 cites W2460134072 @default.
• W3165292893 cites W2463195069 @default.
• W3165292893 cites W2466033010 @default.
• W3165292893 cites W2467159148 @default.
• W3165292893 cites W2510973356 @default.
• W3165292893 cites W2547330766 @default.
• W3165292893 cites W2611717748 @default.
• W3165292893 cites W2623796068 @default.
• W3165292893 cites W2626304656 @default.
• W3165292893 cites W2626725994 @default.
• W3165292893 cites W2737507573 @default.
• W3165292893 cites W2774076172 @default.
• W3165292893 cites W2774876944 @default.
• W3165292893 cites W2782718389 @default.
• W3165292893 cites W2788984248 @default.
• W3165292893 cites W2789422868 @default.
• W3165292893 cites W2792055382 @default.
• W3165292893 cites W2794144380 @default.
• W3165292893 cites W2795066305 @default.
• W3165292893 cites W2891647029 @default.
• W3165292893 cites W2898197535 @default.
• W3165292893 cites W2917101543 @default.
• W3165292893 cites W2960772995 @default.
• W3165292893 cites W2969846817 @default.
• W3165292893 cites W2990321127 @default.
• W3165292893 cites W3205691430 @default.
• W3165292893 cites W4254687493 @default.
• W3165292893 cites W614733133 @default.
• W3165292893 cites W4235220814 @default.
• W3165292893 doi "https://doi.org/10.1038/s41598-021-89907-9" @default.
• W3165292893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8149451" @default.
• W3165292893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34035320" @default.
• W3165292893 hasPublicationYear "2021" @default.
• W3165292893 type Work @default.
• W3165292893 sameAs 3165292893 @default.
• W3165292893 citedByCount "2" @default.
• W3165292893 countsByYear W31652928932021 @default.
• W3165292893 countsByYear W31652928932023 @default.
• W3165292893 crossrefType "journal-article" @default.
• W3165292893 hasAuthorship W3165292893A5033320946 @default.
• W3165292893 hasAuthorship W3165292893A5043638361 @default.
• W3165292893 hasAuthorship W3165292893A5056103070 @default.
• W3165292893 hasAuthorship W3165292893A5058105609 @default.
• W3165292893 hasAuthorship W3165292893A5067604655 @default.
• W3165292893 hasBestOaLocation W31652928931 @default.
• W3165292893 hasConcept C104317684 @default.
• W3165292893 hasConcept C104397665 @default.
• W3165292893 hasConcept C1491633281 @default.
• W3165292893 hasConcept C175369904 @default.
• W3165292893 hasConcept C185592680 @default.
• W3165292893 hasConcept C46111723 @default.
• W3165292893 hasConcept C54355233 @default.
• W3165292893 hasConcept C55493867 @default.
• W3165292893 hasConcept C60644358 @default.
• W3165292893 hasConcept C70721500 @default.
• W3165292893 hasConcept C81885089 @default.
• W3165292893 hasConcept C86803240 @default.
• W3165292893 hasConcept C95444343 @default.
• W3165292893 hasConceptScore W3165292893C104317684 @default.

• next