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• W3165568224 abstract "SUMMARY Having its own genome makes mitochondria a unique and semiautonomous organelle within cells. Mammalian mitochondrial DNA (mtDNA) is double-stranded closed circular molecule of about 16 kb coding 37 genes. Mutations, including deletions in the mitochondrial genome can culminate in different human diseases. Mapping of the deletion junctions suggests that the breakpoints are generally seen at hotspots. ‘9-bp deletion’ (8271-8281), seen in the intergenic region of cytochrome c oxidase II/tRNA Lys , is the most common mitochondrial deletion. While it is associated with several diseases like myopathy, dystonia, and hepatocellular carcinoma, it has also been used as an evolutionary marker. However, the mechanism responsible for its fragility is unclear. In the current study, we show that Endonuclease G, a mitochondrial nuclease responsible for nonspecific cleavage of nuclear DNA during apoptosis, can induce breaks at sequences associated with ‘9-bp deletion’, when it is present on a plasmid or in the mitochondrial genome. Through a series of in vitro and intracellular studies, we show that Endonuclease G binds to G-quadruplex structures formed at the hotspot and induces DNA breaks. Besides, we reconstitute the whole process of ‘9-bp deletion’ using purified Endonuclease G to induce breaks in mtDNA, followed by mitochondrial extract-mediated DSB repair and establish that microhomology-mediated end joining is responsible for the generation of mtDNA deletion. Finally, we show that the whole process is regulated by different stress conditions, which may modulate release of Endonuclease G to the mitochondrial matrix. Therefore, we uncover a new role for Endonuclease G in generating deletions, which is dependent on the formation of G4 DNA within the mitochondrial genome. Thus, in this study we identify a novel property of Endonuclease G, besides its role in apoptosis, and the recently described ‘elimination of paternal mitochondria during fertilization." @default.
• W3165568224 created "2021-06-07" @default.
• W3165568224 creator A5003508566 @default.
• W3165568224 creator A5014148195 @default.
• W3165568224 creator A5024393850 @default.
• W3165568224 creator A5028589689 @default.
• W3165568224 creator A5035638938 @default.
• W3165568224 creator A5038274333 @default.
• W3165568224 creator A5041945521 @default.
• W3165568224 date "2021-05-27" @default.
• W3165568224 modified "2023-09-27" @default.
• W3165568224 title "Unleashing a Novel Function of Endonuclease G in Mitochondrial Genome Instability" @default.
• W3165568224 cites W1450574606 @default.
• W3165568224 cites W1480948328 @default.
• W3165568224 cites W1511523018 @default.
• W3165568224 cites W1536961425 @default.
• W3165568224 cites W1602673445 @default.
• W3165568224 cites W1614924811 @default.
• W3165568224 cites W1650754258 @default.
• W3165568224 cites W1652996689 @default.
• W3165568224 cites W1964135779 @default.
• W3165568224 cites W1967364511 @default.
• W3165568224 cites W1968203430 @default.
• W3165568224 cites W1969625755 @default.
• W3165568224 cites W1970108838 @default.
• W3165568224 cites W1971339396 @default.
• W3165568224 cites W1973687143 @default.
• W3165568224 cites W1975763088 @default.
• W3165568224 cites W1976987687 @default.
• W3165568224 cites W1979240756 @default.
• W3165568224 cites W1986456445 @default.
• W3165568224 cites W1994527193 @default.
• W3165568224 cites W1994815244 @default.
• W3165568224 cites W1998786263 @default.
• W3165568224 cites W2008067403 @default.
• W3165568224 cites W2008441553 @default.
• W3165568224 cites W2008559058 @default.
• W3165568224 cites W2011824034 @default.
• W3165568224 cites W2015934540 @default.
• W3165568224 cites W2020339673 @default.
• W3165568224 cites W2024784932 @default.
• W3165568224 cites W2025907197 @default.
• W3165568224 cites W2026080473 @default.
• W3165568224 cites W2030637794 @default.
• W3165568224 cites W2039323627 @default.
• W3165568224 cites W2042427533 @default.
• W3165568224 cites W2042670577 @default.
• W3165568224 cites W2045214360 @default.
• W3165568224 cites W2054687474 @default.
• W3165568224 cites W2056146464 @default.
• W3165568224 cites W2056375209 @default.
• W3165568224 cites W2066235091 @default.
• W3165568224 cites W2069523941 @default.
• W3165568224 cites W2073921172 @default.
• W3165568224 cites W2074303856 @default.
• W3165568224 cites W2075915930 @default.
• W3165568224 cites W2075927250 @default.
• W3165568224 cites W2077834132 @default.
• W3165568224 cites W2087434357 @default.
• W3165568224 cites W2090837102 @default.
• W3165568224 cites W2093320042 @default.
• W3165568224 cites W2093636220 @default.
• W3165568224 cites W2094071002 @default.
• W3165568224 cites W2094915988 @default.
• W3165568224 cites W2095108895 @default.
• W3165568224 cites W2095468287 @default.
• W3165568224 cites W2099516645 @default.
• W3165568224 cites W2100693064 @default.
• W3165568224 cites W2104198237 @default.
• W3165568224 cites W2107421896 @default.
• W3165568224 cites W2109099295 @default.
• W3165568224 cites W2115638153 @default.
• W3165568224 cites W2117549506 @default.
• W3165568224 cites W2117915375 @default.
• W3165568224 cites W2121454542 @default.
• W3165568224 cites W2126078599 @default.
• W3165568224 cites W2128325937 @default.
• W3165568224 cites W2131561597 @default.
• W3165568224 cites W2134857136 @default.
• W3165568224 cites W2140060651 @default.
• W3165568224 cites W2140499570 @default.
• W3165568224 cites W2142456569 @default.
• W3165568224 cites W2144609528 @default.
• W3165568224 cites W2146979699 @default.
• W3165568224 cites W2154663577 @default.
• W3165568224 cites W2155773930 @default.
• W3165568224 cites W2156875724 @default.
• W3165568224 cites W2159919133 @default.
• W3165568224 cites W2159935796 @default.
• W3165568224 cites W2166664098 @default.
• W3165568224 cites W2167352098 @default.
• W3165568224 cites W2167998990 @default.
• W3165568224 cites W2267919061 @default.
• W3165568224 cites W2272230134 @default.
• W3165568224 cites W2321326379 @default.
• W3165568224 cites W2332723774 @default.
• W3165568224 cites W2343472968 @default.
• W3165568224 cites W2465231113 @default.
• W3165568224 cites W2474238973 @default.
• W3165568224 cites W2548408750 @default.

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