FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3166108768> ?p ?o ?g. }

• W3166108768 abstract "Abstract Background and Aims Three out of four RNA components of ribosomes are encoded by 45S rDNA loci, whose transcripts are processed into 18S, 5.8S and 26S ribosomal RNAs. The loci are organized as long head-to-tail tandem arrays of nearly identical units spanning over several megabases of sequence. Due to this peculiar structure, the number of rRNA genes, their sequence composition and expression status remain unclear, especially in complex polyploid genomes harbouring multiple loci. Here we conducted a complex study to decipher structure and activity of both major and minor rRNA loci in hexaploid bread wheat ( Triticum aestivum ). Methods We employed an original, multi-omics approach, combining chromosome flow sorting and optical mapping with transcriptome and methylome sequencing. Key Results The former two techniques enabled unbiased quantification of rDNA units in particular loci of the wheat genome. Total number of rRNA genes organized in tandem arrays was 4388, with 64.1, 31.4, 3.9 and 0.7% located in short arms of chromosomes 6B, 1B, 5D and 1A, respectively. At the expression level, only 1B and 6B loci contributed to transcription at roughly 2:1 ratio. The 1B:6B ratio varied among five analysed tissues (embryo, coleoptile, root tip, primary leaf, mature leaf), being the highest (2.64:1) in mature leaf and lowest (1.72:1) in coleoptile. Cytosine methylation was considerably higher in CHG contexts in the silenced 5D locus compared to the active 1B and 6B loci. Conclusions A fine genomic organization and tissue-specific expression of rRNA loci were deciphered, for the first time, in a complex polyploid species. We documented various mechanisms of rRNA dosage control, including gene elimination and stable inactivation related to nucleolar subdominance of A and D-genome loci, and a subtle, developmentally regulated silencing of one of the major loci. The results are discussed in the context of wheat evolution and transcription regulation." @default.
• W3166108768 created "2021-06-22" @default.
• W3166108768 creator A5009385040 @default.
• W3166108768 creator A5011548029 @default.
• W3166108768 creator A5030123497 @default.
• W3166108768 creator A5031777650 @default.
• W3166108768 creator A5039340382 @default.
• W3166108768 creator A5060060348 @default.
• W3166108768 creator A5063337128 @default.
• W3166108768 creator A5071812121 @default.
• W3166108768 creator A5074879289 @default.
• W3166108768 creator A5087254133 @default.
• W3166108768 date "2020-08-31" @default.
• W3166108768 modified "2023-10-18" @default.
• W3166108768 title "Anatomy, transcription dynamics and evolution of wheat ribosomal RNA loci deciphered by a multi-omics approach" @default.
• W3166108768 cites W1822731522 @default.
• W3166108768 cites W1873264168 @default.
• W3166108768 cites W1968361512 @default.
• W3166108768 cites W1974832892 @default.
• W3166108768 cites W1990813498 @default.
• W3166108768 cites W1998092685 @default.
• W3166108768 cites W2005187473 @default.
• W3166108768 cites W2007930555 @default.
• W3166108768 cites W2010048498 @default.
• W3166108768 cites W2012012446 @default.
• W3166108768 cites W2013445025 @default.
• W3166108768 cites W2016084883 @default.
• W3166108768 cites W2024730128 @default.
• W3166108768 cites W2025488932 @default.
• W3166108768 cites W2026463142 @default.
• W3166108768 cites W2046181098 @default.
• W3166108768 cites W2046776913 @default.
• W3166108768 cites W2051866625 @default.
• W3166108768 cites W2055220027 @default.
• W3166108768 cites W2074082755 @default.
• W3166108768 cites W2082383416 @default.
• W3166108768 cites W2088180097 @default.
• W3166108768 cites W2093991643 @default.
• W3166108768 cites W2103194389 @default.
• W3166108768 cites W2118744027 @default.
• W3166108768 cites W2126606466 @default.
• W3166108768 cites W2131374955 @default.
• W3166108768 cites W2152192891 @default.
• W3166108768 cites W2155824171 @default.
• W3166108768 cites W2160625611 @default.
• W3166108768 cites W2167499687 @default.
• W3166108768 cites W2170551349 @default.
• W3166108768 cites W2171418340 @default.
• W3166108768 cites W2207536865 @default.
• W3166108768 cites W2227837154 @default.
• W3166108768 cites W2260583560 @default.
• W3166108768 cites W2616584087 @default.
• W3166108768 cites W2886785109 @default.
• W3166108768 cites W2889763942 @default.
• W3166108768 cites W2900108466 @default.
• W3166108768 cites W2945824621 @default.
• W3166108768 cites W2949667635 @default.
• W3166108768 cites W2951212520 @default.
• W3166108768 cites W2951403195 @default.
• W3166108768 cites W2964558489 @default.
• W3166108768 cites W3123381739 @default.
• W3166108768 doi "https://doi.org/10.1101/2020.08.29.273623" @default.
• W3166108768 hasPublicationYear "2020" @default.
• W3166108768 type Work @default.
• W3166108768 sameAs 3166108768 @default.
• W3166108768 citedByCount "4" @default.
• W3166108768 countsByYear W31661087682021 @default.
• W3166108768 countsByYear W31661087682022 @default.
• W3166108768 crossrefType "posted-content" @default.
• W3166108768 hasAuthorship W3166108768A5009385040 @default.
• W3166108768 hasAuthorship W3166108768A5011548029 @default.
• W3166108768 hasAuthorship W3166108768A5030123497 @default.
• W3166108768 hasAuthorship W3166108768A5031777650 @default.
• W3166108768 hasAuthorship W3166108768A5039340382 @default.
• W3166108768 hasAuthorship W3166108768A5060060348 @default.
• W3166108768 hasAuthorship W3166108768A5063337128 @default.
• W3166108768 hasAuthorship W3166108768A5071812121 @default.
• W3166108768 hasAuthorship W3166108768A5074879289 @default.
• W3166108768 hasAuthorship W3166108768A5087254133 @default.
• W3166108768 hasBestOaLocation W31661087681 @default.
• W3166108768 hasConcept C104317684 @default.
• W3166108768 hasConcept C141231307 @default.
• W3166108768 hasConcept C150194340 @default.
• W3166108768 hasConcept C162317418 @default.
• W3166108768 hasConcept C2776626444 @default.
• W3166108768 hasConcept C30481170 @default.
• W3166108768 hasConcept C54355233 @default.
• W3166108768 hasConcept C67905577 @default.
• W3166108768 hasConcept C70721500 @default.
• W3166108768 hasConcept C84597430 @default.
• W3166108768 hasConcept C86803240 @default.
• W3166108768 hasConceptScore W3166108768C104317684 @default.
• W3166108768 hasConceptScore W3166108768C141231307 @default.
• W3166108768 hasConceptScore W3166108768C150194340 @default.
• W3166108768 hasConceptScore W3166108768C162317418 @default.
• W3166108768 hasConceptScore W3166108768C2776626444 @default.
• W3166108768 hasConceptScore W3166108768C30481170 @default.
• W3166108768 hasConceptScore W3166108768C54355233 @default.
• W3166108768 hasConceptScore W3166108768C67905577 @default.
• W3166108768 hasConceptScore W3166108768C70721500 @default.

• next