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- W3166151318 abstract "In studies on the induction of somatic embryogenesis in Opuntia ficus-indica, globular structures were obtained from isolated, in vitro-germinating zygotic embryos in the presence of 0.45×10-6 mol·l-1 2,4-D and 0.91×10-6 or 9.1×10-6 mol·l-1 kinetin. The globuli displayed typical morphological characteristics of embryogenic globuli, i.e., a well-defined globular shape without vascular connection to the original tissue, demonstrating yellowish-white colour. Serial histological analysis during the first 4 weeks of culture revealed that these structures were directly induced after about 2 weeks of primary culture at the periphery, beneath the first cell layers, preferably emerging at subepidermal regions of young cotyledons. Also, a suspensor-like tissue embedding the globuli in the originating material was observed. However, in comparison to young embryonic structures from other species, the globuli from O. ficus-indica displayed atypical cytological characteristics that are related to water management in plants." @default.
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- W3166151318 date "2001-08-27" @default.
- W3166151318 modified "2023-09-23" @default.
- W3166151318 title "Studies on the Induction of Embryogenic Globular Structures in Opuntia ficus-indica" @default.
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