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• W3166303038 abstract "Here we report the 409.5 Mb chromosome-level assembly of the first bred semi-dwarf rice, the Taichung Native 1 (TN1), which served as the template for the development of the Green Revolution (GR) cultivar IR8 miracle rice. We sequenced the TN1 genome utilizing multiple platforms and produced PacBio long reads, Illumina paired-end reads, Illumina mate-pair reads and 10x Genomics linked reads. We used a hybrid approach to assemble the 226× coverage of sequences by a combination of de novo and reference-guided approaches. The assembled TN1 genome has an N50 scaffold size of 33.1 Mb with the longest measuring 45.5 Mb. We annotated 37,526 genes, in which 24,102 (64.23%) were assigned Blast2GO annotations. The genome has 4672 or 95.4% complete BUSCOs and a repeat content of 51.52%. We developed our own method of creating a GR pangenome using the orthologous relationships of the proteins of TN1, IR8, MH63 and IR64, identifying 16,999 core orthologue groups of Green Revolution. From the pangenome, we identified a set of shared and unique gene ontology terms for the accessory clusters, characterizing TN1, IR8, MH63 and IR64. This TN1 genome assembly and GR pangenome will be a resource for new genomic discoveries about Green Revolution, and for improving the disease and insect resistances and the yield of rice." @default.
• W3166303038 created "2021-06-22" @default.
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• W3166303038 date "2021-07-01" @default.
• W3166303038 modified "2023-10-18" @default.
• W3166303038 title "Chromosomal-level genome assembly of the semi-dwarf rice Taichung Native 1, an initiator of Green Revolution" @default.
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• W3166303038 doi "https://doi.org/10.1016/j.ygeno.2021.06.006" @default.
• W3166303038 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34111524" @default.
• W3166303038 hasPublicationYear "2021" @default.
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