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• W3166447089 startingPage "827" @default.
• W3166447089 abstract "Bud rot pathogens cause diseases on Cannabis sativa L. (cannabis, hemp) worldwide through pre- and post-harvest infections of the inflorescence. Seven indoor or outdoor cannabis production sites and three hemp fields were sampled for bud rot and stem canker presence during 2019–2020. Among 178 isolates recovered from diseased tissues, sequences of the ITS1-5.8S-ITS2 region of rDNA, the glyceraldehyde-3-phosphate dehydrogenase (G3PDH) gene and the heat shock 60 (HSP) gene identified the following: Botrytis cinerea (162 isolates), B. pseudocinerea (2), B. porri (1), Sclerotinia sclerotiorum (5), Diaporthe eres (3) and Fusarium graminearum (5). Pathogenicity studies conducted on fresh detached cannabis buds inoculated with spore suspensions or mycelial plugs showed that B. cinerea, S. sclerotiorum and F. graminearum were the most virulent, while B. pseudocinerea, B. porri and D. eres caused significantly less bud rot. Optimal growth of Botrytis species occurred at 15–25°C. In vitro antagonism tests showed that Bacillus spp., Trichoderma asperellum and Gliocladium catenulatum inhibited B. cinerea and S. sclerotiorum colony growth. When applied as a spray 48 h prior to B. cinerea inoculation, all biocontrol agents significantly (P < 0.01) reduced disease development on detached inflorescences. Prolific growth and sporulation of T. asperellum and G. catenulatum were observed on bud tissues. The pathogens B. porri, S. sclerotiorum, D. eres and F. graminearum are described for the first time as cannabis bud rot pathogens. Inoculum from neighbouring fields of diseased garlic, cabbage, blueberry and hay pasture, respectively, likely initiated infection of inflorescences. Several biological control agents show potential for disease reduction through competitive exclusion." @default.
• W3166447089 created "2021-06-22" @default.
• W3166447089 creator A5039480149 @default.
• W3166447089 creator A5075578532 @default.
• W3166447089 date "2021-06-29" @default.
• W3166447089 modified "2023-10-14" @default.
• W3166447089 title "The bud rot pathogens infecting cannabis (<i>Cannabis sativa</i> L., marijuana) inflorescences: symptomology, species identification, pathogenicity and biological control" @default.
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• W3166447089 doi "https://doi.org/10.1080/07060661.2021.1936650" @default.
• W3166447089 hasPublicationYear "2021" @default.
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