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• W316646406 abstract "The effect of dietary supplementation with caprylic acid on the bacterial population of the rabbit caecum and stomach was investigated using two different PCR-based methods: Denaturing Gradient Gel Electrophoresis (DGGE) and Terminal Restriction Fragment Length Polymorphisms (TRFLP). Caprylic acid was added to the diet either in the form of 5 g/kg of the pure acid, or within Akomed R at 10 g/kg (with or without lipase at 10 g/kg). Rabbits fed ad libitum on their respective diets for 27 days before being euthanized and the digestive contents of the caecum and stomach removed for DNA analysis. Neither analytical method suggested a change in the bacterial populations in the stomach dependent on the feeding group of the animal (control, caprylic acid, Akomed R or Akomed R and lipase supplementation). However, the use of DGGE suggested that the caecal samples could be split into two groups; those with no additional fatty acid supplementation of the diet, or where lipase was added along with the fatty acids; and the other where there was caprylic acid added either in its pure form, or in conjunction with other fatty acids in the form of Akomed R supplementation. Furthermore, there was an apparent increase in the detectable biodiversity following supplementation with either caprylic acid or Akomed R. No such segregation between dietary regimes was detected by TRFLP analysis. This is suggestive of there being a shift in relative numbers of specific organisms (DGGE data) without this effect being restricted specifically to one large-scale taxonomic group of organisms (TRFLP data). Thus dietary supplementation with caprylic acid, either in a pure form, or in conjunction with other medium-chain fatty acids, has the ability to target specific groups of microbes, whilst allowing a relatively large-scale bacterial diversity to persist." @default.
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• W316646406 date "2008-01-01" @default.
• W316646406 modified "2023-09-26" @default.
• W316646406 title "Effect of adding dietary caprylic acid on the bacterial population in the rabbit caecum and stomach." @default.
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