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- W3166899052 abstract "We report the design, development, and characterization of a novel multi-spectral fluorescence lifetime measurement device incorporating solid-state detectors and automated gain control. For every excitation pulse (∼1 µJ, 600 ps), this device records complete fluorescence decay from multiple spectral channels simultaneously within microseconds, using a dedicated UV enhanced avalanche photodetector and analog to digital convert (2.5 GS/s) in each channel. Fast (<2 ms) channel-wise dynamic range adjustment maximizes the signal-to-noise ratio. Fluorophores with known lifetime ranging from 0.5-6.0 ns were used to demonstrate the device accuracy. Current results show the clear benefits of this device compared to existing devices employing microchannel-plate photomultiplier tubes. This is demonstrated by 5-fold reduction of lifetime measurement variability in identical conditions, independent gain adjustment in each spectral band, and 4-times faster imaging speed. The use of solid-state detectors will also facilitate future improved performance and miniaturization of the instrument." @default.
- W3166899052 created "2021-06-22" @default.
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- W3166899052 date "2021-06-11" @default.
- W3166899052 modified "2023-10-14" @default.
- W3166899052 title "Multispectral fluorescence lifetime imaging device with a silicon avalanche photodetector" @default.
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- W3166899052 doi "https://doi.org/10.1364/oe.425632" @default.
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