Matches in SemOpenAlex for { <https://semopenalex.org/work/W3167355893> ?p ?o ?g. }
- W3167355893 abstract "Abstract Extracellular vesicles (EVs) are involved in mediating intercellular communication processes. An important goal within the EV field is the study of the biodistribution of EVs and the identification of their target cells. Considering that EV uptake is central for mediating the EVs role in intercellular communication processes, labelling with fluorescent dyes has emerged as a broadly distributed strategy for the identification of the EVs target cells and tissues. However, the accuracy and specificity of commonly utilized labelling dyes has not been sufficiently analyzed. By combining recent advancements in imaging flow cytometry for the phenotypic analysis of single EVs and aiming to identify target cells for EVs within therapeutically relevant MSC-EV preparations, we explored the EV labelling efficacy of various fluorescent dyes, specifically of CFDA-SE, Calcein AM, PKH67, BODIPY-TR-Ceramide and a novel lipid dye named Exoria. Our analyses qualified Exoria as the only dye which specifically labels EVs within our MSC-EV preparations. Furthermore, we demonstrate Exoria labelling does not interfere with the immunomodulatory properties of the MSC-EV preparations as tested in a multi-donor mixed lymphocyte reaction assay. Within this assay, labelled EVs were differentially taken-up by different immune cell types. Overall, our results qualify Exoria as an appropriate dye for the labelling of EVs derived from our MSC-EV preparations, this study also demonstrates the need for the development of next generation EV characterization tools which are able to localize and confirm specificity of EV labelling." @default.
- W3167355893 created "2021-06-22" @default.
- W3167355893 creator A5003845594 @default.
- W3167355893 creator A5031009228 @default.
- W3167355893 creator A5036704352 @default.
- W3167355893 creator A5049046169 @default.
- W3167355893 creator A5050887288 @default.
- W3167355893 creator A5071193568 @default.
- W3167355893 date "2021-06-09" @default.
- W3167355893 modified "2023-09-27" @default.
- W3167355893 title "Imaging flow cytometry challenges the usefulness of classically used EV labelling dyes and qualifies that of a novel dye, named Exoria™ for the labelling of MSC-EV preparations" @default.
- W3167355893 cites W1872071454 @default.
- W3167355893 cites W2004152924 @default.
- W3167355893 cites W2010718864 @default.
- W3167355893 cites W2025612331 @default.
- W3167355893 cites W2033112925 @default.
- W3167355893 cites W2044638539 @default.
- W3167355893 cites W2060387475 @default.
- W3167355893 cites W2096690414 @default.
- W3167355893 cites W2102416146 @default.
- W3167355893 cites W2121354606 @default.
- W3167355893 cites W2141985921 @default.
- W3167355893 cites W2170852633 @default.
- W3167355893 cites W2177788064 @default.
- W3167355893 cites W2259481044 @default.
- W3167355893 cites W2274860869 @default.
- W3167355893 cites W2281182212 @default.
- W3167355893 cites W2301624354 @default.
- W3167355893 cites W2347148183 @default.
- W3167355893 cites W2465243891 @default.
- W3167355893 cites W2552379881 @default.
- W3167355893 cites W2560009252 @default.
- W3167355893 cites W2600094664 @default.
- W3167355893 cites W2612774616 @default.
- W3167355893 cites W2619136320 @default.
- W3167355893 cites W2751524450 @default.
- W3167355893 cites W2755686103 @default.
- W3167355893 cites W2789992655 @default.
- W3167355893 cites W2791527635 @default.
- W3167355893 cites W2808148330 @default.
- W3167355893 cites W2887269406 @default.
- W3167355893 cites W2890739664 @default.
- W3167355893 cites W2897903541 @default.
- W3167355893 cites W2900756811 @default.
- W3167355893 cites W2906563695 @default.
- W3167355893 cites W2913288530 @default.
- W3167355893 cites W2915750135 @default.
- W3167355893 cites W2923606319 @default.
- W3167355893 cites W2931788014 @default.
- W3167355893 cites W2980728936 @default.
- W3167355893 cites W2990529806 @default.
- W3167355893 cites W2998795792 @default.
- W3167355893 cites W3005437667 @default.
- W3167355893 cites W3017233676 @default.
- W3167355893 cites W3025579253 @default.
- W3167355893 cites W3035342952 @default.
- W3167355893 cites W3036417914 @default.
- W3167355893 cites W3048911000 @default.
- W3167355893 cites W3079465195 @default.
- W3167355893 cites W3113127697 @default.
- W3167355893 cites W3116726932 @default.
- W3167355893 cites W3146860356 @default.
- W3167355893 doi "https://doi.org/10.1101/2021.06.09.447567" @default.
- W3167355893 hasPublicationYear "2021" @default.
- W3167355893 type Work @default.
- W3167355893 sameAs 3167355893 @default.
- W3167355893 citedByCount "1" @default.
- W3167355893 countsByYear W31673558932022 @default.
- W3167355893 crossrefType "posted-content" @default.
- W3167355893 hasAuthorship W3167355893A5003845594 @default.
- W3167355893 hasAuthorship W3167355893A5031009228 @default.
- W3167355893 hasAuthorship W3167355893A5036704352 @default.
- W3167355893 hasAuthorship W3167355893A5049046169 @default.
- W3167355893 hasAuthorship W3167355893A5050887288 @default.
- W3167355893 hasAuthorship W3167355893A5071193568 @default.
- W3167355893 hasBestOaLocation W31673558931 @default.
- W3167355893 hasConcept C121332964 @default.
- W3167355893 hasConcept C12554922 @default.
- W3167355893 hasConcept C153911025 @default.
- W3167355893 hasConcept C185592680 @default.
- W3167355893 hasConcept C2776872141 @default.
- W3167355893 hasConcept C2780523633 @default.
- W3167355893 hasConcept C2781374752 @default.
- W3167355893 hasConcept C2992929900 @default.
- W3167355893 hasConcept C41625074 @default.
- W3167355893 hasConcept C52909226 @default.
- W3167355893 hasConcept C553184892 @default.
- W3167355893 hasConcept C55493867 @default.
- W3167355893 hasConcept C62520636 @default.
- W3167355893 hasConcept C70721500 @default.
- W3167355893 hasConcept C86803240 @default.
- W3167355893 hasConcept C91881484 @default.
- W3167355893 hasConcept C95444343 @default.
- W3167355893 hasConceptScore W3167355893C121332964 @default.
- W3167355893 hasConceptScore W3167355893C12554922 @default.
- W3167355893 hasConceptScore W3167355893C153911025 @default.
- W3167355893 hasConceptScore W3167355893C185592680 @default.
- W3167355893 hasConceptScore W3167355893C2776872141 @default.
- W3167355893 hasConceptScore W3167355893C2780523633 @default.
- W3167355893 hasConceptScore W3167355893C2781374752 @default.