Matches in SemOpenAlex for { <https://semopenalex.org/work/W3167492754> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W3167492754 abstract "Airways secrete considerable amounts of acid. In this study we investigated the identity and the pH-dependent function of the apical H channel in the airway epithelium. In pH stat recordings in Ussing chambers, Zn-sensitive acid secretion was activated at a mucosal threshold pH~7, above which it increased pH-dependently at a rate of 339 ± 34 nmole/h.cm per pH unit. Similarly, H currents measured by patch clamping were readily blocked by Zn and activated by an alkaline bath pH. siRNA-mediated knock down of HVCN1 expression in JME cells resulted in total loss of H currents in patch clamp recordings. Cloning of the open reading frame of HVCN1 from primary human airway epithelia resulted in a wild type clone and a mutant clone M91T HVCN1. The activation of acid secretion in epithelia that natively expressed M91T HVCN1 required ~0.5 pH units more alkaline mucosal pH values compared to wild type epithelia. Similarly, activation of H currents across recombinantly expressed M91T HVCN1 required significantly larger membrane depolarization and pH gradients compared to wild type HVCN1. This study provides functional and molecular indications that the HVCN1 H channel mediates pH-regulated acid secretion by the airway epithelium. Funded by CFF and NIH HL86323/HL89196" @default.
- W3167492754 created "2021-06-22" @default.
- W3167492754 creator A5003468273 @default.
- W3167492754 creator A5025869893 @default.
- W3167492754 creator A5086888822 @default.
- W3167492754 creator A5091652058 @default.
- W3167492754 date "2010-04-01" @default.
- W3167492754 modified "2023-09-25" @default.
- W3167492754 title "Function of the HVCN1 proton channel in airway epithelia and a naturally occurring missense mutation, M91T" @default.
- W3167492754 doi "https://doi.org/10.1096/fasebj.24.1_supplement.612.5" @default.
- W3167492754 hasPublicationYear "2010" @default.
- W3167492754 type Work @default.
- W3167492754 sameAs 3167492754 @default.
- W3167492754 citedByCount "0" @default.
- W3167492754 crossrefType "journal-article" @default.
- W3167492754 hasAuthorship W3167492754A5003468273 @default.
- W3167492754 hasAuthorship W3167492754A5025869893 @default.
- W3167492754 hasAuthorship W3167492754A5086888822 @default.
- W3167492754 hasAuthorship W3167492754A5091652058 @default.
- W3167492754 hasConcept C104317684 @default.
- W3167492754 hasConcept C12554922 @default.
- W3167492754 hasConcept C143065580 @default.
- W3167492754 hasConcept C147944092 @default.
- W3167492754 hasConcept C153911025 @default.
- W3167492754 hasConcept C170493617 @default.
- W3167492754 hasConcept C178790620 @default.
- W3167492754 hasConcept C181911157 @default.
- W3167492754 hasConcept C185592680 @default.
- W3167492754 hasConcept C207583985 @default.
- W3167492754 hasConcept C2779932011 @default.
- W3167492754 hasConcept C2781112554 @default.
- W3167492754 hasConcept C3018436504 @default.
- W3167492754 hasConcept C4141045 @default.
- W3167492754 hasConcept C41625074 @default.
- W3167492754 hasConcept C49039625 @default.
- W3167492754 hasConcept C529295009 @default.
- W3167492754 hasConcept C54355233 @default.
- W3167492754 hasConcept C55493867 @default.
- W3167492754 hasConcept C86803240 @default.
- W3167492754 hasConcept C87568996 @default.
- W3167492754 hasConcept C95444343 @default.
- W3167492754 hasConceptScore W3167492754C104317684 @default.
- W3167492754 hasConceptScore W3167492754C12554922 @default.
- W3167492754 hasConceptScore W3167492754C143065580 @default.
- W3167492754 hasConceptScore W3167492754C147944092 @default.
- W3167492754 hasConceptScore W3167492754C153911025 @default.
- W3167492754 hasConceptScore W3167492754C170493617 @default.
- W3167492754 hasConceptScore W3167492754C178790620 @default.
- W3167492754 hasConceptScore W3167492754C181911157 @default.
- W3167492754 hasConceptScore W3167492754C185592680 @default.
- W3167492754 hasConceptScore W3167492754C207583985 @default.
- W3167492754 hasConceptScore W3167492754C2779932011 @default.
- W3167492754 hasConceptScore W3167492754C2781112554 @default.
- W3167492754 hasConceptScore W3167492754C3018436504 @default.
- W3167492754 hasConceptScore W3167492754C4141045 @default.
- W3167492754 hasConceptScore W3167492754C41625074 @default.
- W3167492754 hasConceptScore W3167492754C49039625 @default.
- W3167492754 hasConceptScore W3167492754C529295009 @default.
- W3167492754 hasConceptScore W3167492754C54355233 @default.
- W3167492754 hasConceptScore W3167492754C55493867 @default.
- W3167492754 hasConceptScore W3167492754C86803240 @default.
- W3167492754 hasConceptScore W3167492754C87568996 @default.
- W3167492754 hasConceptScore W3167492754C95444343 @default.
- W3167492754 hasFunder F4320332161 @default.
- W3167492754 hasIssue "S1" @default.
- W3167492754 hasLocation W31674927541 @default.
- W3167492754 hasOpenAccess W3167492754 @default.
- W3167492754 hasPrimaryLocation W31674927541 @default.
- W3167492754 hasRelatedWork W1933239126 @default.
- W3167492754 hasRelatedWork W1987659250 @default.
- W3167492754 hasRelatedWork W2044036281 @default.
- W3167492754 hasRelatedWork W2073090149 @default.
- W3167492754 hasRelatedWork W2077256529 @default.
- W3167492754 hasRelatedWork W2089889685 @default.
- W3167492754 hasRelatedWork W2096648121 @default.
- W3167492754 hasRelatedWork W2101038305 @default.
- W3167492754 hasRelatedWork W2424274959 @default.
- W3167492754 hasRelatedWork W2473463100 @default.
- W3167492754 hasVolume "24" @default.
- W3167492754 isParatext "false" @default.
- W3167492754 isRetracted "false" @default.
- W3167492754 magId "3167492754" @default.
- W3167492754 workType "article" @default.