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• W3167497536 endingPage "2690" @default.
• W3167497536 startingPage "2682" @default.
• W3167497536 abstract "The performance of biosensors is often optimized in buffers, which brings inconsistencies during applications with biological samples. Current strategies for minimizing sample (matrix) interference are complex to automate and miniaturize, involving, e.g., sample dilution or recovery of serum/plasma. This study shows the first systematic analysis using hundreds of actual microfluidic immunoassay fluoropolymer strips to understand matrix interference in microflow systems. As many interfering factors are assay-specific, we have explored matrix interference for a range of enzymatic immunoassays, including a direct mIgG/anti-mIgG, a sandwich cancer biomarker PSA, and a sandwich inflammatory cytokine IL-1β. Serum matrix interference was significantly affected by capillary antibody surface coverage, suggesting for the first time that the main cause of the serum matrix effect is low-affinity serum components (e.g., autoantibodies) competing with high-affinity antigens for the immobilized antibody. Additional experiments carried out with different capillary diameters confirmed the importance of antibody surface coverage in managing matrix interference. Building on these findings, we propose a novel analytical approach where antibody surface coverage and sample incubation times are key for eliminating and/or minimizing serum matrix interference, consisting in bioassay optimization carried out in serum instead of buffer, without compromising the performance of the bioassay or adding extra cost or steps. This will help establishing a new route toward faster development of modern point-of-care tests and effective biosensor development." @default.
• W3167497536 created "2021-06-22" @default.
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• W3167497536 date "2021-06-17" @default.
• W3167497536 modified "2023-09-25" @default.
• W3167497536 title "Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays" @default.
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• W3167497536 doi "https://doi.org/10.1021/acssensors.1c00704" @default.
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• W3167497536 hasPublicationYear "2021" @default.
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