Matches in SemOpenAlex for { <https://semopenalex.org/work/W3167557279> ?p ?o ?g. }
- W3167557279 abstract "Single-molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules are computationally localized from diffraction-limited image sequences and the localizations are used to generate a super-resolution image or a time course of super-resolution images, or to define molecular trajectories. In this Primer, we introduce the basic principles of SMLM techniques before describing the main experimental considerations when performing SMLM, including fluorescent labelling, sample preparation, hardware requirements and image acquisition in fixed and live cells. We then explain how low-resolution image sequences are computationally processed to reconstruct super-resolution images and/or extract quantitative information, and highlight a selection of biological discoveries enabled by SMLM and closely related methods. We discuss some of the main limitations and potential artefacts of SMLM, as well as ways to alleviate them. Finally, we present an outlook on advanced techniques and promising new developments in the fast-evolving field of SMLM. We hope that this Primer will be a useful reference for both newcomers and practitioners of SMLM. This Primer explains the central concepts of single-molecule localization microscopy (SMLM) before discussing experimental considerations regarding fluorophores, optics and data acquisition, processing and analysis. The Primer further describes recent high-impact discoveries made by SMLM techniques and concludes by discussing emerging methodologies." @default.
- W3167557279 created "2021-06-22" @default.
- W3167557279 creator A5006887252 @default.
- W3167557279 creator A5014389184 @default.
- W3167557279 creator A5019498945 @default.
- W3167557279 creator A5022179389 @default.
- W3167557279 creator A5028729865 @default.
- W3167557279 creator A5036041282 @default.
- W3167557279 creator A5052548866 @default.
- W3167557279 creator A5070184235 @default.
- W3167557279 creator A5082193758 @default.
- W3167557279 creator A5091391918 @default.
- W3167557279 date "2021-06-03" @default.
- W3167557279 modified "2023-10-18" @default.
- W3167557279 title "Single-molecule localization microscopy" @default.
- W3167557279 cites W1557847840 @default.
- W3167557279 cites W1782900133 @default.
- W3167557279 cites W1833667256 @default.
- W3167557279 cites W1959369787 @default.
- W3167557279 cites W1969103349 @default.
- W3167557279 cites W1971144253 @default.
- W3167557279 cites W1973274811 @default.
- W3167557279 cites W1975188073 @default.
- W3167557279 cites W1977971917 @default.
- W3167557279 cites W1978532747 @default.
- W3167557279 cites W1985646440 @default.
- W3167557279 cites W1985756888 @default.
- W3167557279 cites W1987900501 @default.
- W3167557279 cites W1990917547 @default.
- W3167557279 cites W1991815226 @default.
- W3167557279 cites W1992858116 @default.
- W3167557279 cites W1993647922 @default.
- W3167557279 cites W1995143570 @default.
- W3167557279 cites W1995311028 @default.
- W3167557279 cites W1996375666 @default.
- W3167557279 cites W1996657515 @default.
- W3167557279 cites W1997107435 @default.
- W3167557279 cites W1997904982 @default.
- W3167557279 cites W1998992455 @default.
- W3167557279 cites W1999085806 @default.
- W3167557279 cites W2001022761 @default.
- W3167557279 cites W2002595364 @default.
- W3167557279 cites W2004324104 @default.
- W3167557279 cites W2004851762 @default.
- W3167557279 cites W2007155950 @default.
- W3167557279 cites W2007971802 @default.
- W3167557279 cites W2008778576 @default.
- W3167557279 cites W2010519082 @default.
- W3167557279 cites W2014786857 @default.
- W3167557279 cites W2015258075 @default.
- W3167557279 cites W2016281621 @default.
- W3167557279 cites W2016675469 @default.
- W3167557279 cites W2020982888 @default.
- W3167557279 cites W2021810362 @default.
- W3167557279 cites W2030224529 @default.
- W3167557279 cites W2030994365 @default.
- W3167557279 cites W2032742734 @default.
- W3167557279 cites W2034713721 @default.
- W3167557279 cites W2035702698 @default.
- W3167557279 cites W2041611886 @default.
- W3167557279 cites W2042205155 @default.
- W3167557279 cites W2043709234 @default.
- W3167557279 cites W2043891370 @default.
- W3167557279 cites W2044010000 @default.
- W3167557279 cites W2048608623 @default.
- W3167557279 cites W2050668820 @default.
- W3167557279 cites W2051365199 @default.
- W3167557279 cites W2053697885 @default.
- W3167557279 cites W2054455698 @default.
- W3167557279 cites W2056963450 @default.
- W3167557279 cites W2058612331 @default.
- W3167557279 cites W2060177007 @default.
- W3167557279 cites W2060187044 @default.
- W3167557279 cites W2061659286 @default.
- W3167557279 cites W2062409614 @default.
- W3167557279 cites W2062552264 @default.
- W3167557279 cites W2064173118 @default.
- W3167557279 cites W2064416009 @default.
- W3167557279 cites W2064833530 @default.
- W3167557279 cites W2065490663 @default.
- W3167557279 cites W2066521860 @default.
- W3167557279 cites W2068098140 @default.
- W3167557279 cites W2070142943 @default.
- W3167557279 cites W2070353646 @default.
- W3167557279 cites W2073576660 @default.
- W3167557279 cites W2074977018 @default.
- W3167557279 cites W2077746083 @default.
- W3167557279 cites W2079748290 @default.
- W3167557279 cites W2080479776 @default.
- W3167557279 cites W2083136325 @default.
- W3167557279 cites W2085767196 @default.
- W3167557279 cites W2087850846 @default.
- W3167557279 cites W2092591295 @default.
- W3167557279 cites W2093861156 @default.
- W3167557279 cites W2094203004 @default.
- W3167557279 cites W2095719211 @default.
- W3167557279 cites W2100453710 @default.
- W3167557279 cites W2100991326 @default.
- W3167557279 cites W2101336703 @default.
- W3167557279 cites W2103241853 @default.