FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3167632754> ?p ?o ?g. }

• W3167632754 abstract "Abstract DNA metabarcoding approaches to analyse complex mixtures of pollen has become the standard in pollination biology, especially in the light of recent threats affecting pollination. In spite of the increasing adoption of High Throughput Sequencing (HTS) approaches, these studies generate huge numbers of raw reads, some of which might be associated to false positives or infrequently recorded species with potentially little biological information. If these reads are not discarded (i.e. pruned), they can lead to changes in the ecological findings and lead to biased conclusions. In this study we reviewed 42 papers in the recent pollen DNA metabarcoding literature and focused on the type of pruning applied. We also tested whether the different types of those cut off threshold may leave a mark on the DNA metabarcoding data. To do so, we compared for the first time community composition, species richness and networks of species interactions (i.e. Connectace, Modularity, Connectivity and Shannon entropy) associated with the most relevant ways of treating HTS outputs: no cut (no reads filtering), or cutting levels obtained as proportional 1% of sample total reads, or as fixed amount of 100 reads, or from ROC (Receiver operator characteristic). Results clearly indicated that pruning type shapes species composition and that to apply or not a threshold dramatically impacts ecological indices, potentially increasing the risk of misinterpreting DNA metabarcoding data under an ecological point of view. Given the high methodological heterogeneity from the revised literature, we discuss in what conditions filtering types may be more appropriate, and also recommend to biologically justify the pruning threshold when analysing DNA metabarcoding raw reads, and to develop shared approaches to make future studies more comparable." @default.
• W3167632754 created "2021-06-22" @default.
• W3167632754 creator A5021169648 @default.
• W3167632754 creator A5032411080 @default.
• W3167632754 creator A5070261490 @default.
• W3167632754 creator A5088827837 @default.
• W3167632754 date "2021-06-15" @default.
• W3167632754 modified "2023-09-27" @default.
• W3167632754 title "Harnessing the power of metabarcoding in the ecological interpretation of plant-pollinator DNA data: strategies and consequences of reads filtering" @default.
• W3167632754 cites W1926731616 @default.
• W3167632754 cites W2029501014 @default.
• W3167632754 cites W2052057004 @default.
• W3167632754 cites W2060257436 @default.
• W3167632754 cites W2076735709 @default.
• W3167632754 cites W2082300488 @default.
• W3167632754 cites W2085277477 @default.
• W3167632754 cites W2099356765 @default.
• W3167632754 cites W2106778108 @default.
• W3167632754 cites W2111641487 @default.
• W3167632754 cites W2115387350 @default.
• W3167632754 cites W2120048439 @default.
• W3167632754 cites W2122184339 @default.
• W3167632754 cites W2122296228 @default.
• W3167632754 cites W2136661588 @default.
• W3167632754 cites W2142359658 @default.
• W3167632754 cites W2142467558 @default.
• W3167632754 cites W2149415648 @default.
• W3167632754 cites W2163250554 @default.
• W3167632754 cites W2167102435 @default.
• W3167632754 cites W2205059992 @default.
• W3167632754 cites W2295147117 @default.
• W3167632754 cites W2334419518 @default.
• W3167632754 cites W2337603118 @default.
• W3167632754 cites W2338899252 @default.
• W3167632754 cites W2474124041 @default.
• W3167632754 cites W2591829732 @default.
• W3167632754 cites W2592367925 @default.
• W3167632754 cites W2593321701 @default.
• W3167632754 cites W2600715883 @default.
• W3167632754 cites W2613921126 @default.
• W3167632754 cites W2623549608 @default.
• W3167632754 cites W2625175743 @default.
• W3167632754 cites W2724989966 @default.
• W3167632754 cites W2740453096 @default.
• W3167632754 cites W2751279829 @default.
• W3167632754 cites W2790714213 @default.
• W3167632754 cites W2792577983 @default.
• W3167632754 cites W2799184652 @default.
• W3167632754 cites W2800305992 @default.
• W3167632754 cites W2801781301 @default.
• W3167632754 cites W2803797147 @default.
• W3167632754 cites W2810540820 @default.
• W3167632754 cites W2866458126 @default.
• W3167632754 cites W2887512826 @default.
• W3167632754 cites W2892073408 @default.
• W3167632754 cites W2897529595 @default.
• W3167632754 cites W2912959159 @default.
• W3167632754 cites W2943747632 @default.
• W3167632754 cites W2944776562 @default.
• W3167632754 cites W2946822048 @default.
• W3167632754 cites W2951242028 @default.
• W3167632754 cites W2952272889 @default.
• W3167632754 cites W2952640720 @default.
• W3167632754 cites W2955255429 @default.
• W3167632754 cites W2955392112 @default.
• W3167632754 cites W2962029563 @default.
• W3167632754 cites W2966267419 @default.
• W3167632754 cites W2978662843 @default.
• W3167632754 cites W2983059328 @default.
• W3167632754 cites W3013949074 @default.
• W3167632754 cites W3018553093 @default.
• W3167632754 cites W3029582435 @default.
• W3167632754 cites W3034406819 @default.
• W3167632754 cites W3047849757 @default.
• W3167632754 cites W3091955378 @default.
• W3167632754 cites W3092454105 @default.
• W3167632754 cites W3093726871 @default.
• W3167632754 cites W3098217199 @default.
• W3167632754 cites W3107449316 @default.
• W3167632754 cites W3108245685 @default.
• W3167632754 cites W3111316607 @default.
• W3167632754 cites W3118490395 @default.
• W3167632754 cites W3118989516 @default.
• W3167632754 cites W3123850793 @default.
• W3167632754 cites W3126274844 @default.
• W3167632754 cites W3127014572 @default.
• W3167632754 cites W3128521950 @default.
• W3167632754 cites W3135209332 @default.
• W3167632754 cites W3152506708 @default.
• W3167632754 cites W3154993755 @default.
• W3167632754 cites W3156882161 @default.
• W3167632754 cites W4210774164 @default.
• W3167632754 cites W4242702784 @default.
• W3167632754 cites W4254851009 @default.
• W3167632754 doi "https://doi.org/10.1101/2021.06.14.448412" @default.
• W3167632754 hasPublicationYear "2021" @default.
• W3167632754 type Work @default.
• W3167632754 sameAs 3167632754 @default.
• W3167632754 citedByCount "0" @default.
• W3167632754 crossrefType "posted-content" @default.

• next