FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3168574761> ?p ?o ?g. }

• W3168574761 endingPage "99" @default.
• W3168574761 startingPage "91" @default.
• W3168574761 abstract "Abstract Background Joint replacement surgery provides articular cartilage samples for chondrocyte isolation. To our knowledge, the effect of the collagenase type on releasing of chondrocytes from the extracellular matrix of cartilage is not reported. Objectives To determine whether cartilage digested with collagenase IA yielded more chondrocytes than that digested with collagenase II and determine whether chondrocytes isolated with collagenase IA could be cultured in vitro. Methods Cartilage slices collected from 18 elderly patients who received joint replacement surgery (16 hips, 2 knees) were digested sequentially with 0.4% pronase E and 0.02% collagenase IA, or with 0.15% collagenase II alone, or sequentially with 0.4% pronase E and 0.02% collagenase II. We compared cell yield from each method. Cell viability by the most effective method was calculated and plotted. The morphology of cultured monolayer chondrocytes was recorded with a light microscope. Results Sequential digestion with pronase E and collagenase IA yielded 2566 ± 873 chondrocytes per mg wet cartilage, which was more effective than the other isolation methods ( P = 0.018). The average chondrocyte viability could reach 84% ± 8% (n = 11). Light microscopic images showed typical chondrocyte morphology in monolayer cultures. Conclusion Sequential digestion of human articular cartilage with pronase E and collagenase IA was more effective than collagenase II alone or collagenase II combined with pronase E for releasing chondrocytes from extracellular matrix of cartilage. Chondrocytes isolated with this method could be maintained in monolayer cultures for at least 2 passages with unaltered morphology." @default.
• W3168574761 created "2021-06-22" @default.
• W3168574761 creator A5020601988 @default.
• W3168574761 creator A5024539931 @default.
• W3168574761 creator A5035440542 @default.
• W3168574761 creator A5051294455 @default.
• W3168574761 creator A5068819901 @default.
• W3168574761 creator A5073833526 @default.
• W3168574761 creator A5087424945 @default.
• W3168574761 date "2021-04-01" @default.
• W3168574761 modified "2023-10-04" @default.
• W3168574761 title "Primary culture of chondrocytes after collagenase IA or II treatment of articular cartilage from elderly patients undergoing arthroplasty" @default.
• W3168574761 cites W1487036716 @default.
• W3168574761 cites W1603799464 @default.
• W3168574761 cites W1807617234 @default.
• W3168574761 cites W1840392162 @default.
• W3168574761 cites W1988592246 @default.
• W3168574761 cites W1992905836 @default.
• W3168574761 cites W2068140578 @default.
• W3168574761 cites W2073456348 @default.
• W3168574761 cites W2074098728 @default.
• W3168574761 cites W2075942323 @default.
• W3168574761 cites W2086898489 @default.
• W3168574761 cites W2088107060 @default.
• W3168574761 cites W2089261882 @default.
• W3168574761 cites W2100240790 @default.
• W3168574761 cites W2105783818 @default.
• W3168574761 cites W2114252409 @default.
• W3168574761 cites W2137087836 @default.
• W3168574761 cites W2141871922 @default.
• W3168574761 cites W2164486347 @default.
• W3168574761 cites W2168564229 @default.
• W3168574761 cites W2411466174 @default.
• W3168574761 cites W2476542314 @default.
• W3168574761 cites W2580409198 @default.
• W3168574761 cites W2739278025 @default.
• W3168574761 cites W2902190855 @default.
• W3168574761 cites W2912069300 @default.
• W3168574761 cites W3019489189 @default.
• W3168574761 cites W4237149571 @default.
• W3168574761 cites W4243063297 @default.
• W3168574761 doi "https://doi.org/10.2478/abm-2021-0011" @default.
• W3168574761 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37551401" @default.
• W3168574761 hasPublicationYear "2021" @default.
• W3168574761 type Work @default.
• W3168574761 sameAs 3168574761 @default.
• W3168574761 citedByCount "3" @default.
• W3168574761 countsByYear W31685747612022 @default.
• W3168574761 countsByYear W31685747612023 @default.
• W3168574761 crossrefType "journal-article" @default.
• W3168574761 hasAuthorship W3168574761A5020601988 @default.
• W3168574761 hasAuthorship W3168574761A5024539931 @default.
• W3168574761 hasAuthorship W3168574761A5035440542 @default.
• W3168574761 hasAuthorship W3168574761A5051294455 @default.
• W3168574761 hasAuthorship W3168574761A5068819901 @default.
• W3168574761 hasAuthorship W3168574761A5073833526 @default.
• W3168574761 hasAuthorship W3168574761A5087424945 @default.
• W3168574761 hasBestOaLocation W31685747611 @default.
• W3168574761 hasConcept C105702510 @default.
• W3168574761 hasConcept C142724271 @default.
• W3168574761 hasConcept C153911025 @default.
• W3168574761 hasConcept C181199279 @default.
• W3168574761 hasConcept C185592680 @default.
• W3168574761 hasConcept C189165786 @default.
• W3168574761 hasConcept C202751555 @default.
• W3168574761 hasConcept C204787440 @default.
• W3168574761 hasConcept C2776164576 @default.
• W3168574761 hasConcept C2776708817 @default.
• W3168574761 hasConcept C2778394429 @default.
• W3168574761 hasConcept C2779851118 @default.
• W3168574761 hasConcept C2780340462 @default.
• W3168574761 hasConcept C2780550940 @default.
• W3168574761 hasConcept C2781403057 @default.
• W3168574761 hasConcept C55493867 @default.
• W3168574761 hasConcept C71924100 @default.
• W3168574761 hasConcept C86803240 @default.
• W3168574761 hasConceptScore W3168574761C105702510 @default.
• W3168574761 hasConceptScore W3168574761C142724271 @default.
• W3168574761 hasConceptScore W3168574761C153911025 @default.
• W3168574761 hasConceptScore W3168574761C181199279 @default.
• W3168574761 hasConceptScore W3168574761C185592680 @default.
• W3168574761 hasConceptScore W3168574761C189165786 @default.
• W3168574761 hasConceptScore W3168574761C202751555 @default.
• W3168574761 hasConceptScore W3168574761C204787440 @default.
• W3168574761 hasConceptScore W3168574761C2776164576 @default.
• W3168574761 hasConceptScore W3168574761C2776708817 @default.
• W3168574761 hasConceptScore W3168574761C2778394429 @default.
• W3168574761 hasConceptScore W3168574761C2779851118 @default.
• W3168574761 hasConceptScore W3168574761C2780340462 @default.
• W3168574761 hasConceptScore W3168574761C2780550940 @default.
• W3168574761 hasConceptScore W3168574761C2781403057 @default.
• W3168574761 hasConceptScore W3168574761C55493867 @default.
• W3168574761 hasConceptScore W3168574761C71924100 @default.
• W3168574761 hasConceptScore W3168574761C86803240 @default.
• W3168574761 hasIssue "2" @default.
• W3168574761 hasLocation W31685747611 @default.
• W3168574761 hasLocation W31685747612 @default.
• W3168574761 hasOpenAccess W3168574761 @default.

• next