Matches in SemOpenAlex for { <https://semopenalex.org/work/W3168841390> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W3168841390 abstract "The calcitonin gene related peptide (CGRP) is a 37 amino acid neuropeptide. Its receptor is a heterodimeric complex of calcitonin receptor-like receptor (CLR) – a family B G-protein coupled receptor – and a single-pass transmembrane protein, receptoractivity modifying protein 1 (RAMP1). Here, we identify residues, within the N-terminal extracellular domain (ECD) of CLR, potentially involved in ligand binding.Certain residues presumed to be possible sites of contact for the CGRP were picked from the CLR/RAMP1 ECD crystal structure (PDB 3N7S). Residues were mutated to alanine (A) bysite-directed mutagenesis (QuikChangeTM, Stratagene). Mutants were analysed for their ability to stimulate cAMP and cell surface expression as previously described [1]. All mutants showed reduced potency, though to varying degrees as indicated by their pEC50 values. W69A and D70Ashowed significant reduction in cell surface expression.These findings suggest that these residues are important for the interaction of CGRP with its receptor. W69A and D70A, part of the WDG motif of family B GPCRs, are thought to rather play a role in receptor stability [2]. The data is consistent with CGRP binding in agroove between CLR and RAMP1. This project was supported byAston School of Life and Health Sciences.References1. Barwell J, Conner A & Poyner D (2011) Extracellular loops 1and 3 and their associated transmembrane regions of the calcitonin receptor-like receptor are needed for CGRP receptor function. Biochim Biophys Acta 1813, 1906–1916.2. Kumar S, Pioszak A, Zhang C et al. (2011) Crystal Structure of the PAC1R Extracellular Domain Unifies a Consensus Fold for Hormone Recognition by Class B G-Protein Cou-pled Receptors. PLoS One 6, e19682" @default.
- W3168841390 created "2021-06-22" @default.
- W3168841390 creator A5037984205 @default.
- W3168841390 creator A5082434067 @default.
- W3168841390 creator A5086141578 @default.
- W3168841390 date "2013-07-01" @default.
- W3168841390 modified "2023-09-23" @default.
- W3168841390 title "Identifying key residues important for CGRP binding to its receptor" @default.
- W3168841390 hasPublicationYear "2013" @default.
- W3168841390 type Work @default.
- W3168841390 sameAs 3168841390 @default.
- W3168841390 citedByCount "0" @default.
- W3168841390 crossrefType "journal-article" @default.
- W3168841390 hasAuthorship W3168841390A5037984205 @default.
- W3168841390 hasAuthorship W3168841390A5082434067 @default.
- W3168841390 hasAuthorship W3168841390A5086141578 @default.
- W3168841390 hasConcept C104317684 @default.
- W3168841390 hasConcept C118303440 @default.
- W3168841390 hasConcept C118892022 @default.
- W3168841390 hasConcept C125268873 @default.
- W3168841390 hasConcept C134018914 @default.
- W3168841390 hasConcept C135285700 @default.
- W3168841390 hasConcept C143065580 @default.
- W3168841390 hasConcept C144293060 @default.
- W3168841390 hasConcept C163170386 @default.
- W3168841390 hasConcept C170493617 @default.
- W3168841390 hasConcept C17971392 @default.
- W3168841390 hasConcept C185592680 @default.
- W3168841390 hasConcept C2779627488 @default.
- W3168841390 hasConcept C2779856020 @default.
- W3168841390 hasConcept C28406088 @default.
- W3168841390 hasConcept C515207424 @default.
- W3168841390 hasConcept C55493867 @default.
- W3168841390 hasConcept C65556437 @default.
- W3168841390 hasConcept C86803240 @default.
- W3168841390 hasConcept C92316933 @default.
- W3168841390 hasConceptScore W3168841390C104317684 @default.
- W3168841390 hasConceptScore W3168841390C118303440 @default.
- W3168841390 hasConceptScore W3168841390C118892022 @default.
- W3168841390 hasConceptScore W3168841390C125268873 @default.
- W3168841390 hasConceptScore W3168841390C134018914 @default.
- W3168841390 hasConceptScore W3168841390C135285700 @default.
- W3168841390 hasConceptScore W3168841390C143065580 @default.
- W3168841390 hasConceptScore W3168841390C144293060 @default.
- W3168841390 hasConceptScore W3168841390C163170386 @default.
- W3168841390 hasConceptScore W3168841390C170493617 @default.
- W3168841390 hasConceptScore W3168841390C17971392 @default.
- W3168841390 hasConceptScore W3168841390C185592680 @default.
- W3168841390 hasConceptScore W3168841390C2779627488 @default.
- W3168841390 hasConceptScore W3168841390C2779856020 @default.
- W3168841390 hasConceptScore W3168841390C28406088 @default.
- W3168841390 hasConceptScore W3168841390C515207424 @default.
- W3168841390 hasConceptScore W3168841390C55493867 @default.
- W3168841390 hasConceptScore W3168841390C65556437 @default.
- W3168841390 hasConceptScore W3168841390C86803240 @default.
- W3168841390 hasConceptScore W3168841390C92316933 @default.
- W3168841390 hasOpenAccess W3168841390 @default.
- W3168841390 hasRelatedWork W1915599524 @default.
- W3168841390 hasRelatedWork W1970669459 @default.
- W3168841390 hasRelatedWork W1976010418 @default.
- W3168841390 hasRelatedWork W1982110986 @default.
- W3168841390 hasRelatedWork W1983105839 @default.
- W3168841390 hasRelatedWork W2018872010 @default.
- W3168841390 hasRelatedWork W2072206821 @default.
- W3168841390 hasRelatedWork W2072290554 @default.
- W3168841390 hasRelatedWork W2075021603 @default.
- W3168841390 hasRelatedWork W2112045237 @default.
- W3168841390 hasRelatedWork W2123278426 @default.
- W3168841390 hasRelatedWork W2126856190 @default.
- W3168841390 hasRelatedWork W2161769863 @default.
- W3168841390 hasRelatedWork W2162312300 @default.
- W3168841390 hasRelatedWork W2167055597 @default.
- W3168841390 hasRelatedWork W2276899373 @default.
- W3168841390 hasRelatedWork W2346512405 @default.
- W3168841390 hasRelatedWork W2531166112 @default.
- W3168841390 hasRelatedWork W2735517537 @default.
- W3168841390 hasRelatedWork W2797372290 @default.
- W3168841390 hasVolume "280" @default.
- W3168841390 isParatext "false" @default.
- W3168841390 isRetracted "false" @default.
- W3168841390 magId "3168841390" @default.
- W3168841390 workType "article" @default.