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• W316946411 abstract "AbstractA wide variety of biomarkers have been identified, which distinguish Small Cell Lung Carcinoma (SCLC) cells from Non-SCLC (NSCLC) cells. They include enzymes, hormones, cell surface-structures, as well as DNA and cytogenetic markers. Most SCLCs are identified by their neuroendocrine phenotype, since NSCLCs generally do not express neuroendocrine features.To isolate additional markers for human SCLC, we performed differential hybridisation studies. In an indirect approach, the pituitary controlled process of background adaptation of the South African clawed toad Xenopus laevis was used, since pituitary cells have characteristics in common with neuroendocrine SCLC cells. A number of cDNA clones were obtained which could represent possible markers for neuroendocrine differentiation. One of the cDNA clones appeared to encode the 7B2 protein, a neuroendocrine polypeptide originally isolated from porcine anterior pituitary glands (Hsi et al., 1982). Subsequently, the human 7B2 gene was isolated (Martens, 1988).From Northern blot analysis of primary human lung carcinomas of various histopathological types and corresponding cell lines using 7B2 cDNA as a molecular probe, the 7B2 gene appeared to be a valuable discriminating marker in human lung cancer diagnosis (Roebroek et al., 1989), as summarised in Table I.To enable immunocytochemical analysis of 7B2 expression, experiments were performed to develop anti-7B2 antibodies. Using bacterially produced 7B2 hybrid-proteins, three mouse monoclonal antibodies were raised: MON-100 (IgG3), MON-101 (IgG1) and MON-102 (IgG1). These monoclonals exhibited specificity for recombinant 7B2, since they reacted with three different 7B2 hybrid-proteins and not with the various carrier proteins (van Duijnhoven et al., 1989). Using recombinant DNA technology, epitope-mapping was performed and from these studies it appeared that all three monoclonal antibodies were probably directed against the same epitope within a sixteen amino acid sequence.The anti-7B2 antibodies appeared to be useful in immunoprecipitation, Western blot analysis and in immunohistochemistry of human lung carcinoma cells. Altogether, this makes the anti-7B2 antibodies useful for pathological subclassification of human lung carcinomas. Furthermore, since 7B2 is expressed in a variety of other neuroendocrine tumours, immunohistochemistry using these antibodies can become a valuable tool for diagnosis of neuroendocrine tumours in general.7B2 is present in human plasma and elevation of 7B2 levels was noted in plasma of patients suffering from various endocrine tumours, including SCLC (Iguchi et al., 1989). We are currently developing a radio-immuno-assay to detect 7B2 in plasma and lung fluid of SCLC-patients for monitoring response to therapy and early detection of relapse." @default.
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• W316946411 date "1991-01-01" @default.
• W316946411 modified "2023-09-28" @default.
• W316946411 title "The 7BS protein as marker for neuroendocrine differentiation in human lung cancer" @default.
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