FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3169655745> ?p ?o ?g. }

• W3169655745 abstract "Trypanosoma cruzi is an obligate intracellular hemoflagellate parasite which cause Chagas disease. This neglected tropical disease causes severe morbidity and mortality in endemic regions. 30% of chronically infected people suffer from cardiac disorder. Chagas heart diseasecan lead to sudden death due to cardiac arrhythmias or progressive heart failure. Invasive trypomastigotes released from infected cells can be carried in vascular endothelial system to infect neighboring and distant cells. During the process of cellular infection, the parasite induces host cells to increase the levels of host thrombospondin-1 (TSP-1) to enable the process of infection. TSP-1 plays important roles in the functioning of vascular cells including vascular endothelial cells with important implications in cardiovascular health. Hippo signaling components, including yes-associated protein 1 (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ), which are upstream of TSP-1 have been linked to the pathophysiology of heart disease. The molecular mechanisms by which T. cruzi signals and eventually infect heart endothelial cells remain unknown. To evaluate the importance of TSP-1 expression in heart endothelial cells during the process of T. cruzi infection, we challenge heart endothelial cells prepared from Wild Type and TSP-1 Knockout mouse (MHEC) to invasive T. cruzi trypomastigotes at different time points and evaluated changes in the Hippo signaling cascade using immunoblotting and immunofluorescence assays. We found that the parasite turned off the hippo signaling pathway in TSP-1KO heart endothelial cells. The levels of SAV1 and MOB1A increased to a maximum of 2.70±0.23 and 5.74±1.45 fold at 3 and 6 h, respectively, in TSP-1KO MHEC compared to WT MHEC following parasite challenge. This was accompanied by a significant continuous increase in the nuclear translocation of downstream effector molecule YAP to a maximum mean nuclear fluorescence intensity of 10.14±0.40 at 6 h compared to wild type cells. Besides this, we found that increased nuclear translocated YAP significantly colocalized with the transcription co-activator molecule pan-TEAD with a maximum Pearson's correlation coefficient of 0.51±0.06 at 6 h compared to YAP-Pan-TEAD colocalization in the WT MHEC, which decreased significantly with to a minimum Pearson's correlation coefficient of 0.30±0.01 at 6 h. Our finding suggests that upregulated TSP-1 is essential for the regulation of the hippo signaling pathway during the early phase of infection. These studies advance our understanding of the Host-Parasite molecular interactions occurring between heart endothelial cells and T. cruzi in the presence and absence of TSP-1. Taken together, this further supports our concept that an increase in the level of TSP-1 induced by the parasite is essential for T. cruzi infection and pathogenesis." @default.
• W3169655745 created "2021-06-22" @default.
• W3169655745 creator A5001405352 @default.
• W3169655745 creator A5001996456 @default.
• W3169655745 creator A5030134677 @default.
• W3169655745 creator A5050210974 @default.
• W3169655745 creator A5055333667 @default.
• W3169655745 creator A5062748013 @default.
• W3169655745 creator A5074136819 @default.
• W3169655745 creator A5090368140 @default.
• W3169655745 creator A5090447843 @default.
• W3169655745 date "2021-05-01" @default.
• W3169655745 modified "2023-09-27" @default.
• W3169655745 title "Thrombospondin‐1 Plays an Essential Role in Hippo Signaling During the Early Phase of Trypanosoma cruzi Infection in Heart Endothelial Cells" @default.
• W3169655745 doi "https://doi.org/10.1096/fasebj.2021.35.s1.04150" @default.
• W3169655745 hasPublicationYear "2021" @default.
• W3169655745 type Work @default.
• W3169655745 sameAs 3169655745 @default.
• W3169655745 citedByCount "0" @default.
• W3169655745 crossrefType "journal-article" @default.
• W3169655745 hasAuthorship W3169655745A5001405352 @default.
• W3169655745 hasAuthorship W3169655745A5001996456 @default.
• W3169655745 hasAuthorship W3169655745A5030134677 @default.
• W3169655745 hasAuthorship W3169655745A5050210974 @default.
• W3169655745 hasAuthorship W3169655745A5055333667 @default.
• W3169655745 hasAuthorship W3169655745A5062748013 @default.
• W3169655745 hasAuthorship W3169655745A5074136819 @default.
• W3169655745 hasAuthorship W3169655745A5090368140 @default.
• W3169655745 hasAuthorship W3169655745A5090447843 @default.
• W3169655745 hasConcept C109523444 @default.
• W3169655745 hasConcept C136764020 @default.
• W3169655745 hasConcept C167814343 @default.
• W3169655745 hasConcept C2777625466 @default.
• W3169655745 hasConcept C2780394083 @default.
• W3169655745 hasConcept C41008148 @default.
• W3169655745 hasConcept C502942594 @default.
• W3169655745 hasConcept C54355233 @default.
• W3169655745 hasConcept C55728118 @default.
• W3169655745 hasConcept C71928629 @default.
• W3169655745 hasConcept C86803240 @default.
• W3169655745 hasConcept C95444343 @default.
• W3169655745 hasConcept C99194444 @default.
• W3169655745 hasConceptScore W3169655745C109523444 @default.
• W3169655745 hasConceptScore W3169655745C136764020 @default.
• W3169655745 hasConceptScore W3169655745C167814343 @default.
• W3169655745 hasConceptScore W3169655745C2777625466 @default.
• W3169655745 hasConceptScore W3169655745C2780394083 @default.
• W3169655745 hasConceptScore W3169655745C41008148 @default.
• W3169655745 hasConceptScore W3169655745C502942594 @default.
• W3169655745 hasConceptScore W3169655745C54355233 @default.
• W3169655745 hasConceptScore W3169655745C55728118 @default.
• W3169655745 hasConceptScore W3169655745C71928629 @default.
• W3169655745 hasConceptScore W3169655745C86803240 @default.
• W3169655745 hasConceptScore W3169655745C95444343 @default.
• W3169655745 hasConceptScore W3169655745C99194444 @default.
• W3169655745 hasIssue "S1" @default.
• W3169655745 hasLocation W31696557451 @default.
• W3169655745 hasOpenAccess W3169655745 @default.
• W3169655745 hasPrimaryLocation W31696557451 @default.
• W3169655745 hasRelatedWork W2026412056 @default.
• W3169655745 hasRelatedWork W2028034242 @default.
• W3169655745 hasRelatedWork W2064302786 @default.
• W3169655745 hasRelatedWork W2103068685 @default.
• W3169655745 hasRelatedWork W2105173802 @default.
• W3169655745 hasRelatedWork W2283784115 @default.
• W3169655745 hasRelatedWork W2981896024 @default.
• W3169655745 hasRelatedWork W3001566363 @default.
• W3169655745 hasRelatedWork W3003445346 @default.
• W3169655745 hasRelatedWork W4243279850 @default.
• W3169655745 hasVolume "35" @default.
• W3169655745 isParatext "false" @default.
• W3169655745 isRetracted "false" @default.
• W3169655745 magId "3169655745" @default.
• W3169655745 workType "article" @default.