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• W3169756605 abstract "In large-scale bioprocesses microbes are exposed to heterogeneous substrate availability reducing the overall process performance. A series of deletion strains was constructed from E. coli MG1655 aiming for a robust phenotype in heterogeneous fermentations with transient starvation. Deletion targets were hand-picked based on a list of genes derived from previous large-scale simulation runs. Each gene deletion was conducted on the premise of strict neutrality towards growth parameters in glucose minimal medium. The final strain of the series, named E. coli RM214, was cultivated continuously in an STR-PFR (stirred tank reactor – plug flow reactor) scale-down reactor. The scale-down reactor system simulated repeated passages through a glucose starvation zone. When exposed to nutrient gradients, E. coli RM214 had a significantly lower maintenance coefficient than E. coli MG1655 (Δm s = 0.038 g Glucose /g CDW /h, p < 0.05). In an exemplary protein production scenario E. coli RM214 remained significantly more productive than E. coli MG1655 reaching 44% higher eGFP yield after 28 h of STR-PFR cultivation. This study developed E. coli RM214 as a robust chassis strain and demonstrated the feasibility of engineering microbial hosts for large-scale applications. • E. coli was engineered to withstand starvation stress in large-scale fermentations. • Deletion targets were chosen to reduce unnecessary metabolic demand from differential regulation. • Engineered E. coli had a significantly lower maintenance coefficient when exposed to repeated starvation stimuli. • Engineered E. coli exhibited more stable protein production with significantly higher yield." @default.
• W3169756605 created "2021-06-22" @default.
• W3169756605 creator A5005872785 @default.
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• W3169756605 date "2021-09-01" @default.
• W3169756605 modified "2023-10-14" @default.
• W3169756605 title "Engineering of a robust Escherichia coli chassis and exploitation for large-scale production processes" @default.
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• W3169756605 doi "https://doi.org/10.1016/j.ymben.2021.05.011" @default.
• W3169756605 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34098100" @default.
• W3169756605 hasPublicationYear "2021" @default.
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