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• W3169961939 endingPage "115447" @default.
• W3169961939 startingPage "115447" @default.
• W3169961939 abstract "The abnormal change of thrombin concentration is closely related with various diseases. By using proximity binding-induced DNA network assembled by nonlinear hybridization chain reaction (HCR), we here developed an enzyme-free and ultrasensitive electrochemical strategy for analyzing thrombin in human serums. The binding of the two different aptamers to a thrombin enhanced the local concentration of the aptamers and facilitates the formation of an intact strand. Subsequently, the formed strand caused nonlinear HCR, forming DNA network. The DNA network bound to the capture probes (CP)-modified electrode, producing electrochemical signal of MB. Benefiting from the proximity binding-induced DNA network, the proposed biosensor exhibited a wide linear range from 1.0 pM to 1.0 nM with a low detection limit of 0.56 pM and high specificity. More importantly, the developed enzyme-free electrochemical biosensor has been used to detect thrombin in serum samples with good performance. This makes such a strategy possess potential promise in the application of clinical diagnosis." @default.
• W3169961939 created "2021-06-22" @default.
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• W3169961939 date "2021-08-01" @default.
• W3169961939 modified "2023-09-27" @default.
• W3169961939 title "Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin" @default.
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• W3169961939 doi "https://doi.org/10.1016/j.jelechem.2021.115447" @default.
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