FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3170677135> ?p ?o ?g. }

• W3170677135 endingPage "114211" @default.
• W3170677135 startingPage "114211" @default.
• W3170677135 abstract "A new variant of bluetongue virus serotype 3, BTV3 ITL 2018 (here named: BTV3), was included in serial dilutions in the BT Proficiency Test 2020. Although the OIE-recommended panBTV real time RT-PCR test targeting genome segment 10 (Seg-10) detected this variant, we showed that reverse transcription (RT) at 61 °C instead of 50 °C completely abolished detection. Another Seg-10 panBTV real time RT-PCR test detected BTV3, irrespective of the temperature of RT. In silico validation showed that each of the OIE-recommended PCR primers using IVI-primers contain single mismatches at the -3 position for BTV3. In contrast, WBVR-primers of a second test completely match to the BTV3 variant. Our results suggest that single mismatches caused false negative PCR results for BTV3 at high RT temperature. Indeed, correction of both IVI-primers for BTV3 led to positive results for BTV3 but negative results for all other samples of the BT Proficiency Test 2020. Apparently, variability of the -3 position is sufficient for discriminative PCR detection, although the single mismatch in the IVI-reverse primer was the most important for this phenomenon. Extensive in silico validation showed that targets of both Seg-10 panBTV RT-PCR tests are not completely conserved, and the detailed effect of single mismatches are hard to predict. Therefore, we recommend at least two panBTV RT-PCR tests to minimize the risk of false negatives. Preferably, their PCR targets should be located at completely different and highly conserved regions of the BTV genome to guarantee adequate detection of future BTV infections." @default.
• W3170677135 created "2021-06-22" @default.
• W3170677135 creator A5041304013 @default.
• W3170677135 creator A5062510891 @default.
• W3170677135 date "2021-09-01" @default.
• W3170677135 modified "2023-10-01" @default.
• W3170677135 title "Critical parameters of real time reverse transcription polymerase chain reaction (RT-PCR) diagnostics: Sensitivity and specificity for bluetongue virus" @default.
• W3170677135 cites W1953884753 @default.
• W3170677135 cites W1997410627 @default.
• W3170677135 cites W2005146466 @default.
• W3170677135 cites W2039039941 @default.
• W3170677135 cites W2042578361 @default.
• W3170677135 cites W2060765637 @default.
• W3170677135 cites W2062015251 @default.
• W3170677135 cites W2102263341 @default.
• W3170677135 cites W2121876967 @default.
• W3170677135 cites W2134822561 @default.
• W3170677135 cites W2152189800 @default.
• W3170677135 cites W2165992919 @default.
• W3170677135 cites W2195182957 @default.
• W3170677135 cites W2517951467 @default.
• W3170677135 cites W2791180237 @default.
• W3170677135 cites W2915430345 @default.
• W3170677135 cites W2946161800 @default.
• W3170677135 cites W2970955887 @default.
• W3170677135 doi "https://doi.org/10.1016/j.jviromet.2021.114211" @default.
• W3170677135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34126108" @default.
• W3170677135 hasPublicationYear "2021" @default.
• W3170677135 type Work @default.
• W3170677135 sameAs 3170677135 @default.
• W3170677135 citedByCount "3" @default.
• W3170677135 countsByYear W31706771352022 @default.
• W3170677135 crossrefType "journal-article" @default.
• W3170677135 hasAuthorship W3170677135A5041304013 @default.
• W3170677135 hasAuthorship W3170677135A5062510891 @default.
• W3170677135 hasBestOaLocation W31706771351 @default.
• W3170677135 hasConcept C104317684 @default.
• W3170677135 hasConcept C105580179 @default.
• W3170677135 hasConcept C141231307 @default.
• W3170677135 hasConcept C142724271 @default.
• W3170677135 hasConcept C143660080 @default.
• W3170677135 hasConcept C153911025 @default.
• W3170677135 hasConcept C156719811 @default.
• W3170677135 hasConcept C159047783 @default.
• W3170677135 hasConcept C178790620 @default.
• W3170677135 hasConcept C184112429 @default.
• W3170677135 hasConcept C185592680 @default.
• W3170677135 hasConcept C200658500 @default.
• W3170677135 hasConcept C204787440 @default.
• W3170677135 hasConcept C2522874641 @default.
• W3170677135 hasConcept C2775905019 @default.
• W3170677135 hasConcept C2777563447 @default.
• W3170677135 hasConcept C4692481 @default.
• W3170677135 hasConcept C48023723 @default.
• W3170677135 hasConcept C49105822 @default.
• W3170677135 hasConcept C54355233 @default.
• W3170677135 hasConcept C70721500 @default.
• W3170677135 hasConcept C71924100 @default.
• W3170677135 hasConcept C86803240 @default.
• W3170677135 hasConcept C90583042 @default.
• W3170677135 hasConceptScore W3170677135C104317684 @default.
• W3170677135 hasConceptScore W3170677135C105580179 @default.
• W3170677135 hasConceptScore W3170677135C141231307 @default.
• W3170677135 hasConceptScore W3170677135C142724271 @default.
• W3170677135 hasConceptScore W3170677135C143660080 @default.
• W3170677135 hasConceptScore W3170677135C153911025 @default.
• W3170677135 hasConceptScore W3170677135C156719811 @default.
• W3170677135 hasConceptScore W3170677135C159047783 @default.
• W3170677135 hasConceptScore W3170677135C178790620 @default.
• W3170677135 hasConceptScore W3170677135C184112429 @default.
• W3170677135 hasConceptScore W3170677135C185592680 @default.
• W3170677135 hasConceptScore W3170677135C200658500 @default.
• W3170677135 hasConceptScore W3170677135C204787440 @default.
• W3170677135 hasConceptScore W3170677135C2522874641 @default.
• W3170677135 hasConceptScore W3170677135C2775905019 @default.
• W3170677135 hasConceptScore W3170677135C2777563447 @default.
• W3170677135 hasConceptScore W3170677135C4692481 @default.
• W3170677135 hasConceptScore W3170677135C48023723 @default.
• W3170677135 hasConceptScore W3170677135C49105822 @default.
• W3170677135 hasConceptScore W3170677135C54355233 @default.
• W3170677135 hasConceptScore W3170677135C70721500 @default.
• W3170677135 hasConceptScore W3170677135C71924100 @default.
• W3170677135 hasConceptScore W3170677135C86803240 @default.
• W3170677135 hasConceptScore W3170677135C90583042 @default.
• W3170677135 hasFunder F4320321780 @default.
• W3170677135 hasLocation W31706771351 @default.
• W3170677135 hasOpenAccess W3170677135 @default.
• W3170677135 hasPrimaryLocation W31706771351 @default.
• W3170677135 hasRelatedWork W1972497539 @default.
• W3170677135 hasRelatedWork W1985154731 @default.
• W3170677135 hasRelatedWork W2067557457 @default.
• W3170677135 hasRelatedWork W2354192789 @default.
• W3170677135 hasRelatedWork W2506504176 @default.
• W3170677135 hasRelatedWork W3025384328 @default.
• W3170677135 hasRelatedWork W3107289505 @default.
• W3170677135 hasRelatedWork W3170677135 @default.
• W3170677135 hasRelatedWork W760073987 @default.
• W3170677135 hasRelatedWork W3157978715 @default.

• next