Matches in SemOpenAlex for { <https://semopenalex.org/work/W3170705421> ?p ?o ?g. }
- W3170705421 endingPage "1894" @default.
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- W3170705421 abstract "Objectives: Untreated HIV infection was previously associated with IL-32 overexpression in gut/intestinal epithelial cells (IEC). Here, we explored IL-32 isoform expression in the colon of people with HIV (PWH) receiving antiretroviral therapy (ART) and IL-32 triggers/modulators in IEC. Design: Sigmoid colon biopsies (SCB) and blood were collected from ART-treated PWH (HIV + ART; n = 17; mean age: 56 years; CD4 + cell counts: 679 cells/μl; time on ART: 72 months) and age-matched HIV-uninfected controls (HIV neg ; n = 5). The IEC line HT-29 was used for mechanistic studies. Methods: Cells from SCB and blood were isolated by enzymatic digestion and/or gradient centrifugation. HT-29 cells were exposed to TLR1–9 agonists, TNF-α, IL-17A and HIV. IL-32α/β/γ/D/ε/θ and IL-17A mRNA levels were quantified by real-time RT-PCR. IL-32 protein levels were quantified by ELISA. Results: IL-32β/γ/ε isoform transcripts were detectable in the blood and SCB, with IL-32β mRNA levels being predominantly expressed in both compartments and at significantly higher levels in HIV + ART compared to HIV neg . IL-17A transcripts were only detectable in SCB, with increased IL-17A levels in HIV neg compared with HIV + ART and negatively correlated with IL-32β mRNA levels. IL-32β/γ/ε isoform mRNA were detected in HT-29 cells upon exposure to TNF-α, Poly I:C (TLR3 agonist), Flagellin (TLR-5 agonist) and HIV. IL-17A significantly decreased both IL-32 β/γ/ε mRNA and cell-associated IL-32 protein levels induced upon TNF-α and Poly I:C triggering. Conclusion: We document IL-32 isoforms abundant in the colon of ART-treated PWH and reveal the capacity of the Th17 hallmark cytokine IL-17A to attenuate IL-32 overexpression in a model of inflamed IEC." @default.
- W3170705421 created "2021-06-22" @default.
- W3170705421 creator A5006237101 @default.
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- W3170705421 creator A5046760367 @default.
- W3170705421 creator A5067324604 @default.
- W3170705421 creator A5086885946 @default.
- W3170705421 date "2021-06-07" @default.
- W3170705421 modified "2023-10-12" @default.
- W3170705421 title "Overt IL-32 isoform expression at intestinal level during HIV-1 infection is negatively regulated by IL-17A" @default.
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