FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3171620663> ?p ?o ?g. }

• W3171620663 abstract "Abstract Proteins are responsible for nearly all cell functions throughout cellular life. To date, the molecular functions of hundreds of proteins have been studied as they are critical to cellular processes. Those proteins are varied dramatically at different statuses and differential stages of the cells even in the same tissue. The existing single-cell tools can only analyze dozens of proteins and thus have not been able to fully characterize a cell yet. Herein, we present a single-cell cyclic multiplex in situ tagging (CycMIST) technology that affords the comprehensive functional proteome profiling of single cells. It permits multiple, separate rounds of multiplex assays of the same single cells on a microchip where each round detects 40-50 proteins. A decoding process is followed to assign protein identities and quantify protein detection signals. We demonstrate the technology on a neuron cell line by detecting 182 proteins that includes surface makers, neuron function proteins, neurodegeneration markers, signaling pathway proteins and transcription factors. Further study on 5XFAD mouse, an Alzheimer’s Disease (AD) model, cells validate the utility of our technology which reveals the deep heterogeneity of brain cells. Through comparison with control mouse cells, the differentially expressed proteins in the AD mouse model have been detected. The single-cell CycMIST technology can potentially analyze the entire functional proteome spectrum, and thus it may offer new insights into cell machinery and advance many fields including systems biology, drug discovery, molecular diagnostics, and clinical studies." @default.
• W3171620663 created "2021-06-22" @default.
• W3171620663 creator A5016455244 @default.
• W3171620663 creator A5042241049 @default.
• W3171620663 creator A5057856636 @default.
• W3171620663 creator A5077809514 @default.
• W3171620663 creator A5078806051 @default.
• W3171620663 creator A5084061655 @default.
• W3171620663 date "2021-06-07" @default.
• W3171620663 modified "2023-10-16" @default.
• W3171620663 title "Cyclic Microchip Assay for Measurement of Hundreds of Functional Proteins in Single Neurons" @default.
• W3171620663 cites W1542220056 @default.
• W3171620663 cites W1767380958 @default.
• W3171620663 cites W1973268693 @default.
• W3171620663 cites W1974565082 @default.
• W3171620663 cites W1979792239 @default.
• W3171620663 cites W1980843818 @default.
• W3171620663 cites W1983816589 @default.
• W3171620663 cites W1995576455 @default.
• W3171620663 cites W2006840724 @default.
• W3171620663 cites W2009391236 @default.
• W3171620663 cites W2024626836 @default.
• W3171620663 cites W2025712131 @default.
• W3171620663 cites W2033416556 @default.
• W3171620663 cites W2038344809 @default.
• W3171620663 cites W2061078277 @default.
• W3171620663 cites W2076687510 @default.
• W3171620663 cites W2109178214 @default.
• W3171620663 cites W2128718699 @default.
• W3171620663 cites W2135461398 @default.
• W3171620663 cites W2161859073 @default.
• W3171620663 cites W2170644095 @default.
• W3171620663 cites W2193067168 @default.
• W3171620663 cites W2248179576 @default.
• W3171620663 cites W2406725268 @default.
• W3171620663 cites W2460791123 @default.
• W3171620663 cites W2462492481 @default.
• W3171620663 cites W2520489503 @default.
• W3171620663 cites W2581064244 @default.
• W3171620663 cites W2607386808 @default.
• W3171620663 cites W2763051133 @default.
• W3171620663 cites W2832881433 @default.
• W3171620663 cites W2886232524 @default.
• W3171620663 cites W2887091285 @default.
• W3171620663 cites W2890156321 @default.
• W3171620663 cites W2898512315 @default.
• W3171620663 cites W2922214442 @default.
• W3171620663 cites W2949238013 @default.
• W3171620663 cites W2951821937 @default.
• W3171620663 cites W2952558325 @default.
• W3171620663 cites W2961621566 @default.
• W3171620663 cites W2968292465 @default.
• W3171620663 cites W2968995168 @default.
• W3171620663 cites W2969767806 @default.
• W3171620663 cites W2977152589 @default.
• W3171620663 cites W2985684500 @default.
• W3171620663 cites W3003317013 @default.
• W3171620663 cites W3006533027 @default.
• W3171620663 cites W3047081808 @default.
• W3171620663 cites W4247419735 @default.
• W3171620663 doi "https://doi.org/10.1101/2021.06.06.447288" @default.
• W3171620663 hasPublicationYear "2021" @default.
• W3171620663 type Work @default.
• W3171620663 sameAs 3171620663 @default.
• W3171620663 citedByCount "1" @default.
• W3171620663 countsByYear W31716206632022 @default.
• W3171620663 crossrefType "posted-content" @default.
• W3171620663 hasAuthorship W3171620663A5016455244 @default.
• W3171620663 hasAuthorship W3171620663A5042241049 @default.
• W3171620663 hasAuthorship W3171620663A5057856636 @default.
• W3171620663 hasAuthorship W3171620663A5077809514 @default.
• W3171620663 hasAuthorship W3171620663A5078806051 @default.
• W3171620663 hasAuthorship W3171620663A5084061655 @default.
• W3171620663 hasBestOaLocation W31716206631 @default.
• W3171620663 hasConcept C104317684 @default.
• W3171620663 hasConcept C104397665 @default.
• W3171620663 hasConcept C14036430 @default.
• W3171620663 hasConcept C142724271 @default.
• W3171620663 hasConcept C1491633281 @default.
• W3171620663 hasConcept C2776925932 @default.
• W3171620663 hasConcept C2776950831 @default.
• W3171620663 hasConcept C2779134260 @default.
• W3171620663 hasConcept C2781188995 @default.
• W3171620663 hasConcept C46111723 @default.
• W3171620663 hasConcept C54355233 @default.
• W3171620663 hasConcept C60644358 @default.
• W3171620663 hasConcept C70721500 @default.
• W3171620663 hasConcept C71924100 @default.
• W3171620663 hasConcept C86803240 @default.
• W3171620663 hasConcept C95444343 @default.
• W3171620663 hasConceptScore W3171620663C104317684 @default.
• W3171620663 hasConceptScore W3171620663C104397665 @default.
• W3171620663 hasConceptScore W3171620663C14036430 @default.
• W3171620663 hasConceptScore W3171620663C142724271 @default.
• W3171620663 hasConceptScore W3171620663C1491633281 @default.
• W3171620663 hasConceptScore W3171620663C2776925932 @default.
• W3171620663 hasConceptScore W3171620663C2776950831 @default.
• W3171620663 hasConceptScore W3171620663C2779134260 @default.
• W3171620663 hasConceptScore W3171620663C2781188995 @default.
• W3171620663 hasConceptScore W3171620663C46111723 @default.

• next