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- W3172872521 abstract "One of the characteristic features of metastatic breast cancer is increased cellular storage of neutral lipid in cytoplasmic lipid droplets (CLDs). CLD accumulation is associated with increased cancer aggressiveness, suggesting CLDs contribute to metastasis. However, how CLDs contribute to metastasis is not clear. CLDs are composed of a neutral lipid core, a phospholipid monolayer, and associated proteins. Proteins that associate with CLDs regulate both cellular and CLD metabolism; however, the proteome of CLDs in metastatic breast cancer and how these proteins may contribute to breast cancer progression is unknown. Therefore, the purpose of this study was to identify the proteome and assess the characteristics of CLDs in the MCF10CA1a human metastatic breast cancer cell line. Utilizing shotgun proteomics, we identified over 1500 proteins involved in a variety of cellular processes in the isolated CLD fraction. Interestingly, unlike other cell lines such as adipocytes or enterocytes, the most enriched protein categories were involved in cellular processes outside of lipid metabolism. For example, cell-cell adhesion was the most enriched category of proteins identified, and many of these proteins have been implicated in breast cancer metastasis. In addition, we characterized CLD size and area in MCF10CA1a cells using transmission electron microscopy. Our results provide a hypothesis-generating list of potential players in breast cancer progression and offers a new perspective on the role of CLDs in cancer." @default.
- W3172872521 created "2021-06-22" @default.
- W3172872521 creator A5022569995 @default.
- W3172872521 creator A5026812062 @default.
- W3172872521 creator A5028888879 @default.
- W3172872521 creator A5051823031 @default.
- W3172872521 date "2021-06-01" @default.
- W3172872521 modified "2023-09-27" @default.
- W3172872521 title "Proteomic Characterization of Cytoplasmic Lipid Droplets in Human Metastatic Breast Cancer Cells" @default.
- W3172872521 cites W1543903063 @default.
- W3172872521 cites W155775833 @default.
- W3172872521 cites W1635741485 @default.
- W3172872521 cites W1860208758 @default.
- W3172872521 cites W1949196050 @default.
- W3172872521 cites W1969251489 @default.
- W3172872521 cites W1974730258 @default.
- W3172872521 cites W1977145129 @default.
- W3172872521 cites W1982452656 @default.
- W3172872521 cites W1994390460 @default.
- W3172872521 cites W1996575904 @default.
- W3172872521 cites W1997238838 @default.
- W3172872521 cites W2006437070 @default.
- W3172872521 cites W2007387279 @default.
- W3172872521 cites W2012004008 @default.
- W3172872521 cites W2013697921 @default.
- W3172872521 cites W2016010488 @default.
- W3172872521 cites W2022650450 @default.
- W3172872521 cites W2027204269 @default.
- W3172872521 cites W2029889940 @default.
- W3172872521 cites W2031540681 @default.
- W3172872521 cites W2036321220 @default.
- W3172872521 cites W2037150053 @default.
- W3172872521 cites W2038893299 @default.
- W3172872521 cites W2039303083 @default.
- W3172872521 cites W2039926571 @default.
- W3172872521 cites W2040679153 @default.
- W3172872521 cites W2044073767 @default.
- W3172872521 cites W2045983090 @default.
- W3172872521 cites W2057511144 @default.
- W3172872521 cites W2061795728 @default.
- W3172872521 cites W2067065072 @default.
- W3172872521 cites W2068050893 @default.
- W3172872521 cites W2069151390 @default.
- W3172872521 cites W2069194455 @default.
- W3172872521 cites W2073057074 @default.
- W3172872521 cites W2077474820 @default.
- W3172872521 cites W2080752012 @default.
- W3172872521 cites W2084177233 @default.
- W3172872521 cites W2094093571 @default.
- W3172872521 cites W2099540110 @default.
- W3172872521 cites W2103117581 @default.
- W3172872521 cites W2106690639 @default.
- W3172872521 cites W2118013260 @default.
- W3172872521 cites W2119023084 @default.
- W3172872521 cites W2119050497 @default.
- W3172872521 cites W2122143508 @default.
- W3172872521 cites W2127694087 @default.
- W3172872521 cites W2128159578 @default.
- W3172872521 cites W2132489811 @default.
- W3172872521 cites W2133465414 @default.
- W3172872521 cites W2135688339 @default.
- W3172872521 cites W2147833903 @default.
- W3172872521 cites W2158217645 @default.
- W3172872521 cites W2161397941 @default.
- W3172872521 cites W2163301096 @default.
- W3172872521 cites W2165200432 @default.
- W3172872521 cites W2166446773 @default.
- W3172872521 cites W2166910049 @default.
- W3172872521 cites W2170776626 @default.
- W3172872521 cites W2220777476 @default.
- W3172872521 cites W2222632618 @default.
- W3172872521 cites W2265836483 @default.
- W3172872521 cites W2289129784 @default.
- W3172872521 cites W2331313517 @default.
- W3172872521 cites W2333156716 @default.
- W3172872521 cites W2337088385 @default.
- W3172872521 cites W2340125363 @default.
- W3172872521 cites W2464007823 @default.
- W3172872521 cites W2558655274 @default.
- W3172872521 cites W2560888990 @default.
- W3172872521 cites W2582655559 @default.
- W3172872521 cites W2625629561 @default.
- W3172872521 cites W2626003366 @default.
- W3172872521 cites W2732331423 @default.
- W3172872521 cites W2737660124 @default.
- W3172872521 cites W2739442195 @default.
- W3172872521 cites W2743619083 @default.
- W3172872521 cites W2744906292 @default.
- W3172872521 cites W2749065334 @default.
- W3172872521 cites W2767754396 @default.
- W3172872521 cites W2769980254 @default.
- W3172872521 cites W2788543154 @default.
- W3172872521 cites W2792039826 @default.
- W3172872521 cites W2795702337 @default.
- W3172872521 cites W2803653486 @default.
- W3172872521 cites W2806073287 @default.
- W3172872521 cites W2807903845 @default.
- W3172872521 cites W2884515763 @default.
- W3172872521 cites W2885582506 @default.
- W3172872521 cites W2886385224 @default.