Matches in SemOpenAlex for { <https://semopenalex.org/work/W3173134052> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W3173134052 abstract "In situ imaging of molecular markers on a physical chromosome is an indispensable tool for refin-ing of genetic maps and validation genome assembly at the chromosomal level. Despite tremen-dous progress in genome sequencing the plant genome assembly at chromosome level still remain a challenge. Recently developed optical and Hi-C mapping aim to assist in genome assembly. For high-confidence in the genome assembly at chromosome level more independent approaches will be required. The present study aimed to refined an ultrasensitive Tyr-FISH technique and to de-velop a reliable and easy method for in situ mapping of a short unique DNA sequences on plant chromosomes. We have carefully analyzed the critical steps of the Tyr-FISH technique to find out the reasons for the failures of using the method. It has been shown that successful visualization of marker/gene depends significantly on method of chromosome slide preparation, probe design and labelling, high stringency washing. Appropriate adjustment of these steps allowed us to detect a short DNA sequence of 1.7Kb with a frequency of 51.6%. Based on our results, we developed a reliable and simple protocol for dual-color Tyr-FISH visualization of short unique DNA sequences on plant chromosomes. New protocol allows more accurate determination of the physical distance between markers and can be applied for faster integration of genetic and cytogenetic maps." @default.
- W3173134052 created "2021-06-22" @default.
- W3173134052 creator A5004085923 @default.
- W3173134052 creator A5017593920 @default.
- W3173134052 creator A5027531653 @default.
- W3173134052 creator A5032790799 @default.
- W3173134052 creator A5035746877 @default.
- W3173134052 creator A5036715802 @default.
- W3173134052 creator A5048404504 @default.
- W3173134052 date "2021-04-22" @default.
- W3173134052 modified "2023-09-23" @default.
- W3173134052 title "A Dual-Color Tyr-FISH Method for Visualizing Genes/Markers on Plant Chromosomes to Create Integrated Genetic and Cytogenetic Maps" @default.
- W3173134052 doi "https://doi.org/10.20944/preprints202104.0607.v1" @default.
- W3173134052 hasPublicationYear "2021" @default.
- W3173134052 type Work @default.
- W3173134052 sameAs 3173134052 @default.
- W3173134052 citedByCount "0" @default.
- W3173134052 crossrefType "posted-content" @default.
- W3173134052 hasAuthorship W3173134052A5004085923 @default.
- W3173134052 hasAuthorship W3173134052A5017593920 @default.
- W3173134052 hasAuthorship W3173134052A5027531653 @default.
- W3173134052 hasAuthorship W3173134052A5032790799 @default.
- W3173134052 hasAuthorship W3173134052A5035746877 @default.
- W3173134052 hasAuthorship W3173134052A5036715802 @default.
- W3173134052 hasAuthorship W3173134052A5048404504 @default.
- W3173134052 hasBestOaLocation W31731340521 @default.
- W3173134052 hasConcept C104317684 @default.
- W3173134052 hasConcept C122735190 @default.
- W3173134052 hasConcept C124101348 @default.
- W3173134052 hasConcept C141231307 @default.
- W3173134052 hasConcept C2909208804 @default.
- W3173134052 hasConcept C30481170 @default.
- W3173134052 hasConcept C36464697 @default.
- W3173134052 hasConcept C41008148 @default.
- W3173134052 hasConcept C505870484 @default.
- W3173134052 hasConcept C51679486 @default.
- W3173134052 hasConcept C54355233 @default.
- W3173134052 hasConcept C70721500 @default.
- W3173134052 hasConcept C86803240 @default.
- W3173134052 hasConceptScore W3173134052C104317684 @default.
- W3173134052 hasConceptScore W3173134052C122735190 @default.
- W3173134052 hasConceptScore W3173134052C124101348 @default.
- W3173134052 hasConceptScore W3173134052C141231307 @default.
- W3173134052 hasConceptScore W3173134052C2909208804 @default.
- W3173134052 hasConceptScore W3173134052C30481170 @default.
- W3173134052 hasConceptScore W3173134052C36464697 @default.
- W3173134052 hasConceptScore W3173134052C41008148 @default.
- W3173134052 hasConceptScore W3173134052C505870484 @default.
- W3173134052 hasConceptScore W3173134052C51679486 @default.
- W3173134052 hasConceptScore W3173134052C54355233 @default.
- W3173134052 hasConceptScore W3173134052C70721500 @default.
- W3173134052 hasConceptScore W3173134052C86803240 @default.
- W3173134052 hasLocation W31731340521 @default.
- W3173134052 hasLocation W31731340522 @default.
- W3173134052 hasOpenAccess W3173134052 @default.
- W3173134052 hasPrimaryLocation W31731340521 @default.
- W3173134052 hasRelatedWork W1442603624 @default.
- W3173134052 hasRelatedWork W1866648957 @default.
- W3173134052 hasRelatedWork W1892590436 @default.
- W3173134052 hasRelatedWork W2012748577 @default.
- W3173134052 hasRelatedWork W2031436818 @default.
- W3173134052 hasRelatedWork W2119103177 @default.
- W3173134052 hasRelatedWork W2151912589 @default.
- W3173134052 hasRelatedWork W2779613469 @default.
- W3173134052 hasRelatedWork W2975451038 @default.
- W3173134052 hasRelatedWork W2094069166 @default.
- W3173134052 isParatext "false" @default.
- W3173134052 isRetracted "false" @default.
- W3173134052 magId "3173134052" @default.
- W3173134052 workType "article" @default.