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- W3173275846 abstract "Triton X-100 (TX-100) is a non-ionic detergent widely used for bioassay studies. We have used electrophysiological and functional techniques to examine the effects of TX-100 on L-type voltage operated calcium channel (VOCC) activity in vascular smooth muscle cells (VSMCs), cardiac myocytes and pancreatic β-cells, cell types in which these channels play a critical functional role. TX-100 (1.6 μM) reversibly inhibited (p<0.01) currents due to expression of recombinant L-type VOCCs (CaV1.2) reducing peak currents as compared to control. TX-100 (1.6 μM) also reversibly inhibited (p<0.01) native L-type VOCC currents from rat VSMCs and cardiac myocytes, and murine and human pancreatic β-cells reducing peak currents as compared to control. In functional studies, TX-100 (165 nM-3.4 μM) caused concentration-dependent relaxation of rat mesenteric arteries independent of the endothelium. TX-100 (1.6 μM) also inhibited depolarization-induced exocytosis in murine and human isolated pancreatic β-cells. These data indicate that low micromolar concentrations of TX-100 significantly inhibit L-type VOCC activity in a number of cell types, an effect paralleled by inhibition of cell functions dependent upon activation of these channels. As this inhibitory effect occurs at concentrations below those used to solubilize proteins and lipophilic agents, it may preclude the use of TX-100 in some bioassays." @default.
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- W3173275846 date "2012-04-01" @default.
- W3173275846 modified "2023-09-28" @default.
- W3173275846 title "Triton X‐100 inhibits L‐type voltage‐operated calcium channels" @default.
- W3173275846 doi "https://doi.org/10.1096/fasebj.26.1_supplement.1115.15" @default.
- W3173275846 hasPublicationYear "2012" @default.
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