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- W3173408405 abstract "This study aimed to determine CD4 count from cryopreservation of Buffy coat (BC) and Peripheral Blood Mononuclear Cell (PBMC) with and without ficoll. Fifteen EDTA Blood sample (2 ml for each tube) were drawn from one adult healthy subject. The samples were categorized into five group before measuring the CD4 level (which were fresh whole blood [Group(G)-I], BC without ficoll [fresh and frozen ] , and PBMC resulted from BC and ficoll isolation [fresh and frozen ]. Each group was replicated three times. Blood storage before preparation was less than four hours. Two months cryopreservation using liquid nitrogen (in 40% FBS, 10% DMSO, and RPMI) was conducted. The mean value of CD4 count (cell /?l) were 522 (G-I), 1410 (G-II), 906 (G-III), 807 (G-IV), and 733 (G-V). CD4 count, after 2 month preservation in liquid nitrogen, of the BC sample (G -III) was higher (906 cell /?l ) than PBMC (G-IV) sample (733 cell /?l).Keywords: CD4, Buffy coat, PBMC, Cryopreservation" @default.
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- W3173408405 date "2015-04-01" @default.
- W3173408405 modified "2023-09-24" @default.
- W3173408405 title "CD4 count from cryopreservation of buffy coat and pheripheral blood mononuclear cells" @default.
- W3173408405 hasPublicationYear "2015" @default.
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