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- W3173442783 abstract "Nucleic acid-based systems play important roles in antiviral defense, including CRISPR/Cas that adopts RNA-guided DNA cleavage to prevent DNA phage infection and RNA interference (RNAi) that employs RNA-guided RNA cleavage to defend against RNA virus infection. Here, we report a novel type of nucleic acid-based antiviral system that exists in mouse embryonic stem cells (mESCs), which suppresses RNA virus infection by DNA-mediated RNA cleavage. We found that the viral RNA of encephalomyocarditis virus can be reverse transcribed into complementary DNA (vcDNA) by the reverse transcriptase (RTase) encoded by endogenous retrovirus-like elements in mESCs. The vcDNA is negative-sense single-stranded and forms DNA/RNA hybrid with viral RNA. The viral RNA in the heteroduplex is subsequently destroyed by cellular RNase H1, leading to robust suppression of viral growth. Furthermore, either inhibition of the RTase activity or depletion of endogenous RNase H1 results in the promotion of virus proliferation. Altogether, our results provide intriguing insights into the antiviral mechanism of mESCs and the antiviral function of endogenized retroviruses and cellular RNase H. Such a natural nucleic acid-based antiviral mechanism in mESCs is referred to as ERASE (endogenous RTase/RNase H-mediated antiviral system), which is an addition to the previously known nucleic acid-based antiviral mechanisms including CRISPR/Cas in bacteria and RNAi in plants and invertebrates." @default.
- W3173442783 created "2021-07-05" @default.
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- W3173442783 date "2021-06-22" @default.
- W3173442783 modified "2023-10-14" @default.
- W3173442783 title "Endogenous reverse transcriptase and RNase H-mediated antiviral mechanism in embryonic stem cells" @default.
- W3173442783 cites W1551725457 @default.
- W3173442783 cites W1557728494 @default.
- W3173442783 cites W1846100490 @default.
- W3173442783 cites W1944771638 @default.
- W3173442783 cites W1953890763 @default.
- W3173442783 cites W1960895270 @default.
- W3173442783 cites W1965281176 @default.
- W3173442783 cites W1967706132 @default.
- W3173442783 cites W1968772442 @default.
- W3173442783 cites W1975643185 @default.
- W3173442783 cites W1976736675 @default.
- W3173442783 cites W1977771685 @default.
- W3173442783 cites W1985688174 @default.
- W3173442783 cites W1986447756 @default.
- W3173442783 cites W1987480613 @default.
- W3173442783 cites W2003171404 @default.
- W3173442783 cites W2009603499 @default.
- W3173442783 cites W2011861577 @default.
- W3173442783 cites W2014542764 @default.
- W3173442783 cites W2024893223 @default.
- W3173442783 cites W2027795157 @default.
- W3173442783 cites W2029467634 @default.
- W3173442783 cites W2031211461 @default.
- W3173442783 cites W2038484136 @default.
- W3173442783 cites W2039382895 @default.
- W3173442783 cites W2040891039 @default.
- W3173442783 cites W2047032063 @default.
- W3173442783 cites W2055508399 @default.
- W3173442783 cites W2057061886 @default.
- W3173442783 cites W2057648499 @default.
- W3173442783 cites W2058012269 @default.
- W3173442783 cites W2059919630 @default.
- W3173442783 cites W2063669037 @default.
- W3173442783 cites W2064815984 @default.
- W3173442783 cites W2068878293 @default.
- W3173442783 cites W2074897380 @default.
- W3173442783 cites W2078403259 @default.
- W3173442783 cites W2079894383 @default.
- W3173442783 cites W2080409511 @default.
- W3173442783 cites W2082944610 @default.
- W3173442783 cites W2090260381 @default.
- W3173442783 cites W2099610218 @default.
- W3173442783 cites W2100361299 @default.
- W3173442783 cites W2102372281 @default.
- W3173442783 cites W2127999459 @default.
- W3173442783 cites W2139269736 @default.
- W3173442783 cites W2141245361 @default.
- W3173442783 cites W2142396207 @default.
- W3173442783 cites W2150368381 @default.
- W3173442783 cites W2151464048 @default.
- W3173442783 cites W2159086136 @default.
- W3173442783 cites W2161222316 @default.
- W3173442783 cites W2168909179 @default.
- W3173442783 cites W2255369131 @default.
- W3173442783 cites W2283404274 @default.
- W3173442783 cites W2318777795 @default.
- W3173442783 cites W2399422472 @default.
- W3173442783 cites W2406557042 @default.
- W3173442783 cites W2509346014 @default.
- W3173442783 cites W2527879866 @default.
- W3173442783 cites W2604845045 @default.
- W3173442783 cites W2755096673 @default.
- W3173442783 cites W2755157156 @default.
- W3173442783 cites W2772147257 @default.
- W3173442783 cites W2795531081 @default.
- W3173442783 cites W2809237949 @default.
- W3173442783 cites W2887464566 @default.
- W3173442783 cites W2895829909 @default.
- W3173442783 cites W2896401613 @default.
- W3173442783 cites W2898198088 @default.
- W3173442783 cites W2904072383 @default.
- W3173442783 cites W2936448388 @default.
- W3173442783 cites W3039815223 @default.
- W3173442783 cites W3081228941 @default.
- W3173442783 cites W3096234081 @default.
- W3173442783 cites W4212766227 @default.