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- W3173644959 abstract "Protein fossils, i.e. noncoding DNA descended from coding DNA, arise frequently from transposable elements (TEs), decayed genes, and viral integrations. They can reveal, and mislead about, evolutionary history and relationships. They have been detected by comparing DNA to protein sequences, but current methods are not optimized for this task. We describe a powerful DNA-protein homology search method. We use a 64 (times ) 21 substitution matrix, which is fitted to sequence data, automatically learning the genetic code. We detect subtly homologous regions by considering alternative possible alignments between them, and calculate significance (probability of occurring by chance between random sequences). Our method detects TE protein fossils much more sensitively than blastx, and ({>}10{times }) faster. Of the (sim )7 major categories of eukaryotic TE, three were long thought absent in mammals: we find two of them in the human genome, polinton and DIRS/Ngaro. This method increases our power to find ancient fossils, and perhaps to detect non-standard genetic codes. The alternative-alignments and significance paradigm is not specific to DNA-protein comparison, and could benefit homology search generally." @default.
- W3173644959 created "2021-07-05" @default.
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- W3173644959 date "2021-01-01" @default.
- W3173644959 modified "2023-10-09" @default.
- W3173644959 title "Improved DNA-versus-Protein Homology Search for Protein Fossils" @default.
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- W3173644959 doi "https://doi.org/10.1007/978-3-030-74432-8_11" @default.
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