FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3173784107> ?p ?o ?g. }

• W3173784107 abstract "Does natural variation exist in the endometrial stem/progenitor cell and protein composition of menstrual fluid across menstrual cycles in women?Limited variation exists in the percentage of some endometrial stem/progenitor cell types and abundance of selected proteins in menstrual fluid within and between a cohort of women.Menstrual fluid is a readily available biofluid that can represent the endometrial environment, containing endometrial stem/progenitor cells and protein factors. It is unknown whether there is natural variation in the cellular and protein content across menstrual cycles of individual women, which has significant implications for the use of menstrual fluid in research and clinical applications.Menstrual fluid was collected from 11 non-pregnant females with regular menstrual cycles. Participants had not used hormonal medications in the previous 3 months. Participants collected menstrual fluid samples from up to five cycles using a silicone menstrual cup worn on Day 2 of menstrual bleeding.Menstrual fluid samples were centrifuged to separate soluble proteins and cells. Cells were depleted of red blood cells and CD45+ leucocytes. Menstrual fluid-derived endometrial stem/progenitor cells were characterized using multicolour flow cytometry including markers for endometrial stem/progenitor cells N-cadherin (NCAD) and stage-specific embryonic antigen-1 (SSEA-1) (for endometrial epithelial progenitor cells; eEPC), and sushi domain containing-2 (SUSD2) (for endometrial mesenchymal stem cells; eMSC). The clonogenicity of menstrual fluid-derived endometrial cells was assessed using colony forming unit assays. Menstrual fluid supernatant was analyzed using a custom magnetic Luminex assay.Endometrial stem/progenitor cells are shed in menstrual fluid and demonstrate clonogenic properties. The intraparticipant agreement for SUSD2+ menstrual fluid-derived eMSC (MF-eMSC), SSEA-1+ and NCAD+SSEA-1+ MF-eEPC, and stromal clonogenicity were moderate-good (intraclass correlation; ICC: 0.75, 0.56, 0.54 and 0.52, respectively), indicating limited variability across menstrual cycles. Endometrial inflammatory and repair proteins were detectable in menstrual fluid supernatant, with five of eight (63%) factors demonstrating moderate intraparticipant agreement (secretory leukocyte protein inhibitor (SLPI), lipocalin-2 (NGAL), lactoferrin, follistatin-like 1 (FSTL1), human epididymis protein-4 (HE4); ICC ranges: 0.57-0.69). Interparticipant variation was limited for healthy participants, with the exception of key outliers of which some had self-reported menstrual pathologies.N/A. There are no OMICS or other data sets relevant to this study.The main limitations to this research relate to the difficulty of obtaining menstrual fluid samples across multiple menstrual cycles in a consistent manner. Several participants could only donate across <3 cycles and the duration of wearing the menstrual cup varied between 4 and 6 h within and between women. Due to the limited sample size used in this study, wider studies involving multiple consecutive menstrual cycles and a larger cohort of women will be required to fully determine the normal range of endometrial stem/progenitor cell and supernatant protein content of menstrual fluid. Possibility for selection bias and true representation of the population of women should also be considered.Menstrual fluid is a reliable source of endometrial stem/progenitor cells and related endometrial proteins with diagnostic potential. The present study indicates that a single menstrual sample may be sufficient in characterizing a variety of cellular and protein parameters across women's menstrual cycles. The results also demonstrate the potential of menstrual fluid for identifying endometrial and menstrual abnormalities in both research and clinical settings as a non-invasive method for assessing endometrial health.This study was supported by grants from the Australian National Health and Medical Research Council to C.E.G. (Senior Research Fellowship 1024298 and Investigator Fellowship 1173882) and to J.E. (project grant 1047756), the Monash IVF Research Foundation to C.E.G. and the Victorian Government's Operational Infrastructure Support Program. K.A.W., M.L.D.-T., S.G.S. and J.E. declare no conflicts of interest. C.E.G. reports grants from NHMRC, during the conduct of the study; grants from EndoFound USA, grants from Ferring Research Innovation, grants from United States Department of Defence, grants from Clue-Utopia Research Foundation, outside the submitted work. CEF reports grants from EndoFound USA, grants from Clue-Utopia Research Foundation, outside the submitted work." @default.
• W3173784107 created "2021-07-05" @default.
• W3173784107 creator A5025262411 @default.
• W3173784107 creator A5032226344 @default.
• W3173784107 creator A5044939352 @default.
• W3173784107 creator A5046840933 @default.
• W3173784107 creator A5074346695 @default.
• W3173784107 creator A5078027324 @default.
• W3173784107 date "2021-06-26" @default.
• W3173784107 modified "2023-10-09" @default.
• W3173784107 title "Menstrual fluid endometrial stem/progenitor cell and supernatant protein content: cyclical variation and indicative range" @default.
• W3173784107 cites W1488482313 @default.
• W3173784107 cites W1596171810 @default.
• W3173784107 cites W1918154392 @default.
• W3173784107 cites W1969760896 @default.
• W3173784107 cites W1983755163 @default.
• W3173784107 cites W1985192557 @default.
• W3173784107 cites W2003403648 @default.
• W3173784107 cites W2012544207 @default.
• W3173784107 cites W2013074450 @default.
• W3173784107 cites W2023644659 @default.
• W3173784107 cites W2035271221 @default.
• W3173784107 cites W2035628574 @default.
• W3173784107 cites W2040708685 @default.
• W3173784107 cites W2041316663 @default.
• W3173784107 cites W2049145125 @default.
• W3173784107 cites W2054629971 @default.
• W3173784107 cites W2062270100 @default.
• W3173784107 cites W2068653318 @default.
• W3173784107 cites W2073187304 @default.
• W3173784107 cites W2083940160 @default.
• W3173784107 cites W2088989332 @default.
• W3173784107 cites W2106270466 @default.
• W3173784107 cites W2106464827 @default.
• W3173784107 cites W2106686929 @default.
• W3173784107 cites W2107668882 @default.
• W3173784107 cites W2109160378 @default.
• W3173784107 cites W2110001173 @default.
• W3173784107 cites W2121759988 @default.
• W3173784107 cites W2128790762 @default.
• W3173784107 cites W2131870029 @default.
• W3173784107 cites W2140368652 @default.
• W3173784107 cites W2143161807 @default.
• W3173784107 cites W2143516059 @default.
• W3173784107 cites W2152065348 @default.
• W3173784107 cites W2153392280 @default.
• W3173784107 cites W2154484611 @default.
• W3173784107 cites W2157162135 @default.
• W3173784107 cites W2171825112 @default.
• W3173784107 cites W2174922077 @default.
• W3173784107 cites W2203749559 @default.
• W3173784107 cites W2211006361 @default.
• W3173784107 cites W2327037637 @default.
• W3173784107 cites W2552861535 @default.
• W3173784107 cites W2593033301 @default.
• W3173784107 cites W2759859589 @default.
• W3173784107 cites W2791278894 @default.
• W3173784107 cites W2794339668 @default.
• W3173784107 cites W2883270717 @default.
• W3173784107 cites W2885069342 @default.
• W3173784107 cites W2904508164 @default.
• W3173784107 cites W2999536987 @default.
• W3173784107 cites W3002932856 @default.
• W3173784107 cites W3025098861 @default.
• W3173784107 cites W3041064744 @default.
• W3173784107 cites W3041501106 @default.
• W3173784107 cites W3100285024 @default.
• W3173784107 cites W3157215790 @default.
• W3173784107 doi "https://doi.org/10.1093/humrep/deab156" @default.
• W3173784107 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34173001" @default.
• W3173784107 hasPublicationYear "2021" @default.
• W3173784107 type Work @default.
• W3173784107 sameAs 3173784107 @default.
• W3173784107 citedByCount "12" @default.
• W3173784107 countsByYear W31737841072021 @default.
• W3173784107 countsByYear W31737841072022 @default.
• W3173784107 countsByYear W31737841072023 @default.
• W3173784107 crossrefType "journal-article" @default.
• W3173784107 hasAuthorship W3173784107A5025262411 @default.
• W3173784107 hasAuthorship W3173784107A5032226344 @default.
• W3173784107 hasAuthorship W3173784107A5044939352 @default.
• W3173784107 hasAuthorship W3173784107A5046840933 @default.
• W3173784107 hasAuthorship W3173784107A5074346695 @default.
• W3173784107 hasAuthorship W3173784107A5078027324 @default.
• W3173784107 hasConcept C126322002 @default.
• W3173784107 hasConcept C134018914 @default.
• W3173784107 hasConcept C16685009 @default.
• W3173784107 hasConcept C201750760 @default.
• W3173784107 hasConcept C2779058012 @default.
• W3173784107 hasConcept C2779742232 @default.
• W3173784107 hasConcept C28328180 @default.
• W3173784107 hasConcept C42407357 @default.
• W3173784107 hasConcept C71315377 @default.
• W3173784107 hasConcept C71924100 @default.
• W3173784107 hasConcept C86803240 @default.
• W3173784107 hasConcept C95444343 @default.
• W3173784107 hasConceptScore W3173784107C126322002 @default.
• W3173784107 hasConceptScore W3173784107C134018914 @default.
• W3173784107 hasConceptScore W3173784107C16685009 @default.
• W3173784107 hasConceptScore W3173784107C201750760 @default.

• next