Matches in SemOpenAlex for { <https://semopenalex.org/work/W3173996961> ?p ?o ?g. }
- W3173996961 endingPage "826" @default.
- W3173996961 startingPage "808" @default.
- W3173996961 abstract "The parasite Trypanosoma brucei cycles between an insect and a mammalian host and is the causative agent of sleeping sickness. Here, we performed high-throughput mapping of pseudouridines (Ψs) on mRNA from two life stages of the parasite. The analysis revealed ~273 Ψs, including developmentally regulated Ψs that are guided by homologs of pseudouridine synthases (PUS1, 3, 5, and 7). Mutating the U that undergoes pseudouridylation in the 3' UTR of valyl-tRNA synthetase destabilized the mRNA level. To investigate the mechanism by which Ψ affects the stability of this mRNA, proteins that bind to the 3' UTR were identified, including the RNA binding protein RBSR1. The binding of RBSR1 protein to the 3' UTR was stronger when lacking Ψ compared to transcripts carrying the modification, suggesting that Ψ can inhibit the binding of proteins to their target and thus affect the stability of mRNAs. Consequently, Ψ modification on mRNA adds an additional level of regulation to the dominant post-transcriptional control in these parasites." @default.
- W3173996961 created "2021-07-05" @default.
- W3173996961 creator A5003516827 @default.
- W3173996961 creator A5005178590 @default.
- W3173996961 creator A5006646678 @default.
- W3173996961 creator A5030887593 @default.
- W3173996961 creator A5048752058 @default.
- W3173996961 creator A5056279786 @default.
- W3173996961 creator A5056692823 @default.
- W3173996961 creator A5072824630 @default.
- W3173996961 creator A5075252986 @default.
- W3173996961 creator A5079904690 @default.
- W3173996961 creator A5087255793 @default.
- W3173996961 date "2021-07-07" @default.
- W3173996961 modified "2023-09-27" @default.
- W3173996961 title "Pseudouridines on <i>Trypanosoma brucei</i> mRNAs are developmentally regulated: Implications to mRNA stability and protein binding" @default.
- W3173996961 cites W1500914927 @default.
- W3173996961 cites W1850578074 @default.
- W3173996961 cites W1970994599 @default.
- W3173996961 cites W1973767731 @default.
- W3173996961 cites W1984195294 @default.
- W3173996961 cites W1987202538 @default.
- W3173996961 cites W1991998643 @default.
- W3173996961 cites W2008121312 @default.
- W3173996961 cites W2014056463 @default.
- W3173996961 cites W2017942151 @default.
- W3173996961 cites W2019427404 @default.
- W3173996961 cites W2021564378 @default.
- W3173996961 cites W2031985733 @default.
- W3173996961 cites W2038841445 @default.
- W3173996961 cites W2058123073 @default.
- W3173996961 cites W2061440884 @default.
- W3173996961 cites W2061671159 @default.
- W3173996961 cites W2077345777 @default.
- W3173996961 cites W2084703994 @default.
- W3173996961 cites W2085928743 @default.
- W3173996961 cites W2087106902 @default.
- W3173996961 cites W2088352540 @default.
- W3173996961 cites W2094113855 @default.
- W3173996961 cites W2097227527 @default.
- W3173996961 cites W2097603183 @default.
- W3173996961 cites W2101327042 @default.
- W3173996961 cites W2102904980 @default.
- W3173996961 cites W2103874817 @default.
- W3173996961 cites W2105219600 @default.
- W3173996961 cites W2109700867 @default.
- W3173996961 cites W2113738713 @default.
- W3173996961 cites W2120937476 @default.
- W3173996961 cites W2125022130 @default.
- W3173996961 cites W2125783737 @default.
- W3173996961 cites W2127405785 @default.
- W3173996961 cites W2132750922 @default.
- W3173996961 cites W2136711098 @default.
- W3173996961 cites W2149876974 @default.
- W3173996961 cites W2153641622 @default.
- W3173996961 cites W2156216634 @default.
- W3173996961 cites W2161890613 @default.
- W3173996961 cites W2187336842 @default.
- W3173996961 cites W2257200896 @default.
- W3173996961 cites W2345999730 @default.
- W3173996961 cites W2395646452 @default.
- W3173996961 cites W2555701464 @default.
- W3173996961 cites W2565793297 @default.
- W3173996961 cites W2571114720 @default.
- W3173996961 cites W2580590280 @default.
- W3173996961 cites W2581823621 @default.
- W3173996961 cites W2604222542 @default.
- W3173996961 cites W2743366849 @default.
- W3173996961 cites W2753241613 @default.
- W3173996961 cites W2786230719 @default.
- W3173996961 cites W2947963640 @default.
- W3173996961 cites W2957700484 @default.
- W3173996961 cites W3014228495 @default.
- W3173996961 cites W3088559610 @default.
- W3173996961 cites W3097320691 @default.
- W3173996961 doi "https://doi.org/10.1111/mmi.14774" @default.
- W3173996961 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34165831" @default.
- W3173996961 hasPublicationYear "2021" @default.
- W3173996961 type Work @default.
- W3173996961 sameAs 3173996961 @default.
- W3173996961 citedByCount "9" @default.
- W3173996961 countsByYear W31739969612021 @default.
- W3173996961 countsByYear W31739969612022 @default.
- W3173996961 countsByYear W31739969612023 @default.
- W3173996961 crossrefType "journal-article" @default.
- W3173996961 hasAuthorship W3173996961A5003516827 @default.
- W3173996961 hasAuthorship W3173996961A5005178590 @default.
- W3173996961 hasAuthorship W3173996961A5006646678 @default.
- W3173996961 hasAuthorship W3173996961A5030887593 @default.
- W3173996961 hasAuthorship W3173996961A5048752058 @default.
- W3173996961 hasAuthorship W3173996961A5056279786 @default.
- W3173996961 hasAuthorship W3173996961A5056692823 @default.
- W3173996961 hasAuthorship W3173996961A5072824630 @default.
- W3173996961 hasAuthorship W3173996961A5075252986 @default.
- W3173996961 hasAuthorship W3173996961A5079904690 @default.
- W3173996961 hasAuthorship W3173996961A5087255793 @default.
- W3173996961 hasBestOaLocation W31739969611 @default.
- W3173996961 hasConcept C104317684 @default.