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- W3173999906 abstract "Although it is known that the relative investment in tracheal volume increases with the size of grasshoppers, S. americana 6th instars are not able to maintain their mass-specific metabolic rate during the intermolt period. Previous work using x-ray synchrotron imaging has shown that tracheal volumes in the abdomen and metathoracic femur are reduced during the intermolt period. While x-ray synchrotron imaging allows live specimens to be analyzed, there are drawbacks to this method: small tracheae are hard to visualize and 3D analysis is complicated. To overcome these problems we used PU4ii [vasQtec, Switzerland], a low viscosity polymer resin, to create corrosion casts of the femoral tracheal system in female 6th instar grasshoppers of varying age. After the resin was injected and allowed to polymerize, tissues were chemically digested, leaving only the tracheal cast. Tracheae in four categories were compared across early, middle, and late stage 6th instars. The categories were: primary tracheae running along the proximodistal axis; secondary tracheae on the dorsoventral axis in a chevron pattern; tertiary tracheae descending from the chevrons into the muscle cavity; and air sacs located at the hip and knee joints. Casts were photographed using light microscopy; approximate tracheae volumes were calculated using Leica Application Suite software. We hypothesize that the relative volume of the tracheal system, as measured by select tracheae and air sac volumes, decreases during the 6th instar stage. Preliminary results show that during the intermolt period, the cross-sectional area of primary tracheae decreases by 61.8% and air sac volumes decrease by 4.7%. Previous work suggests that growing non-respiratory tissue compresses the tracheal system against the rigid cuticular exoskeleton. Funding has been provided by Union College." @default.
- W3173999906 created "2021-07-05" @default.
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- W3173999906 date "2015-04-01" @default.
- W3173999906 modified "2023-10-16" @default.
- W3173999906 title "Understanding Intermolt Tracheal Changes Using Corrosion Casting" @default.
- W3173999906 doi "https://doi.org/10.1096/fasebj.29.1_supplement.686.10" @default.
- W3173999906 hasPublicationYear "2015" @default.
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