FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3174069314> ?p ?o ?g. }

• W3174069314 endingPage "e11619" @default.
• W3174069314 startingPage "e11619" @default.
• W3174069314 abstract "Environmental DNA methods such as metabarcoding have been suggested as possible alternatives or complements to the current practice of morphology-based diversity assessment for characterizing benthic communities in marine sediment. However, the source volume used in sediment eDNA studies is several magnitudes lower than that used in morphological identification. Here, we used data from a North Sea benthic sampling station to investigate to what extent metabarcoding data is affected by sampling bias and spatial heterogeneity. Using three grab parallels, we sampled five separate sediment samples from each grab. We then made five DNA extraction replicates from each sediment sample. Each extract was amplified targeting both the 18S SSU rRNA V1–V2 region for total eukaryotic composition, and the cytochrome c oxidase subunit I (COI) gene for metazoans only. In both datasets, extract replicates from the same sediment sample were significantly more similar than different samples from the same grab. Further, samples from different grabs were less similar than those from the same grab for 18S. Interestingly, this was not true for COI metabarcoding, where the differences within the same grab were similar to the differences between grabs. We also investigated how much of the total identified richness could be covered by extract replicates, individual sediment samples and all sediment samples from a single grab, as well as the variability of Shannon diversity and, for COI, macrofaunal biotic indices indicating environmental status. These results were largely consistent with the beta diversity findings, and show that total eukaryotic diversity can be well represented using 18S metabarcoding with a manageable number of biological replicates. Based on these results, we strongly recommend the combination of different parts of the surface of single grabs for eDNA extraction as well as several grab replicates, or alternatively box cores or similar. This will dilute the effects of dominating species and increase the coverage of alpha diversity. COI-based metabarcoding consistency was found to be lower compared to 18S, but COI macrofauna-based indices were more consistent than direct COI alpha diversity measures." @default.
• W3174069314 created "2021-07-05" @default.
• W3174069314 creator A5005529953 @default.
• W3174069314 creator A5012662200 @default.
• W3174069314 creator A5034786659 @default.
• W3174069314 date "2021-06-21" @default.
• W3174069314 modified "2023-10-15" @default.
• W3174069314 title "Grab what you can—an evaluation of spatial replication to decrease heterogeneity in sediment eDNA metabarcoding" @default.
• W3174069314 cites W1551951435 @default.
• W3174069314 cites W1585980954 @default.
• W3174069314 cites W1905207763 @default.
• W3174069314 cites W1970722670 @default.
• W3174069314 cites W2006282417 @default.
• W3174069314 cites W2026677149 @default.
• W3174069314 cites W2036897871 @default.
• W3174069314 cites W2063008800 @default.
• W3174069314 cites W2083189830 @default.
• W3174069314 cites W2108106320 @default.
• W3174069314 cites W2114859967 @default.
• W3174069314 cites W2131645019 @default.
• W3174069314 cites W2168564982 @default.
• W3174069314 cites W2194764270 @default.
• W3174069314 cites W2212911571 @default.
• W3174069314 cites W227278642 @default.
• W3174069314 cites W2316199253 @default.
• W3174069314 cites W2409209401 @default.
• W3174069314 cites W2412804189 @default.
• W3174069314 cites W2425675749 @default.
• W3174069314 cites W2500544443 @default.
• W3174069314 cites W2513506562 @default.
• W3174069314 cites W2534138766 @default.
• W3174069314 cites W2554050174 @default.
• W3174069314 cites W2565568379 @default.
• W3174069314 cites W2580767461 @default.
• W3174069314 cites W2600954339 @default.
• W3174069314 cites W2626376206 @default.
• W3174069314 cites W2724989966 @default.
• W3174069314 cites W2760845297 @default.
• W3174069314 cites W2765747110 @default.
• W3174069314 cites W2767140392 @default.
• W3174069314 cites W2775206584 @default.
• W3174069314 cites W2801860893 @default.
• W3174069314 cites W2803453656 @default.
• W3174069314 cites W2808622463 @default.
• W3174069314 cites W2810540820 @default.
• W3174069314 cites W2849202658 @default.
• W3174069314 cites W2883984093 @default.
• W3174069314 cites W2886205776 @default.
• W3174069314 cites W2886938767 @default.
• W3174069314 cites W2887756261 @default.
• W3174069314 cites W2888320035 @default.
• W3174069314 cites W2888402234 @default.
• W3174069314 cites W2888451776 @default.
• W3174069314 cites W2943747632 @default.
• W3174069314 cites W2952463830 @default.
• W3174069314 cites W2965120276 @default.
• W3174069314 cites W2969600503 @default.
• W3174069314 cites W2982085983 @default.
• W3174069314 cites W3009603309 @default.
• W3174069314 cites W3021648506 @default.
• W3174069314 cites W3027396750 @default.
• W3174069314 cites W3029234950 @default.
• W3174069314 cites W3030077075 @default.
• W3174069314 cites W3093122654 @default.
• W3174069314 cites W3094776990 @default.
• W3174069314 cites W3111285500 @default.
• W3174069314 cites W3121953277 @default.
• W3174069314 doi "https://doi.org/10.7717/peerj.11619" @default.
• W3174069314 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8223902" @default.
• W3174069314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34221724" @default.
• W3174069314 hasPublicationYear "2021" @default.
• W3174069314 type Work @default.
• W3174069314 sameAs 3174069314 @default.
• W3174069314 citedByCount "9" @default.
• W3174069314 countsByYear W31740693142021 @default.
• W3174069314 countsByYear W31740693142022 @default.
• W3174069314 countsByYear W31740693142023 @default.
• W3174069314 crossrefType "journal-article" @default.
• W3174069314 hasAuthorship W3174069314A5005529953 @default.
• W3174069314 hasAuthorship W3174069314A5012662200 @default.
• W3174069314 hasAuthorship W3174069314A5034786659 @default.
• W3174069314 hasBestOaLocation W31740693141 @default.
• W3174069314 hasConcept C106131492 @default.
• W3174069314 hasConcept C130217890 @default.
• W3174069314 hasConcept C140779682 @default.
• W3174069314 hasConcept C141732470 @default.
• W3174069314 hasConcept C151730666 @default.
• W3174069314 hasConcept C18903297 @default.
• W3174069314 hasConcept C2779969263 @default.
• W3174069314 hasConcept C2816523 @default.
• W3174069314 hasConcept C30968088 @default.
• W3174069314 hasConcept C31972630 @default.
• W3174069314 hasConcept C41008148 @default.
• W3174069314 hasConcept C53565203 @default.
• W3174069314 hasConcept C62648534 @default.
• W3174069314 hasConcept C83042747 @default.
• W3174069314 hasConcept C86803240 @default.
• W3174069314 hasConceptScore W3174069314C106131492 @default.

• next