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- W3174306489 abstract "Epulopiscium sp. Type B (Epulo) is a large symbiotic aquatic bacterium that resides in the gut of surgeon fish. From genomic data, Epulo is predicted to have a chemotactic sensory and motility system similar to Escherichia coli and Bacillus subtilis. Our experiments are designed to test the protein-protein interactions between methyl accepting chemotaxis proteins (MCPs) and the Che proteins, CheA (a sensory kinase) and CheW (a coupling protein), through the use of a yeast-2-hybrid (Y2H) genetic assay. We hypothesize based on genetic homology that Epulo chemotaxis proteins will behave in a similar fashion to their orthologs in B.subtilis and E.coli. The Y2H tests protein-protein interactions through metabolic and drug selection in the yeast Saccharomyces cerevisiae. The genes in question; MCP3, MCP6 and CheW will be cloned into pGADT7 vector while CheA will be inserted into pGBKT7. Preliminary results confirmed an interaction between CheA and CheW; other interactions have yet to be detected; possibly because CheW is mildly toxic to the yeast strain Y187. Alternatively, a CheA+CheW+MCP ternary complex may be necessary for MCP binding. This research is funded by a Hartwick College Faculty Research Grant awarded to AJP." @default.
- W3174306489 created "2021-07-05" @default.
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- W3174306489 date "2013-04-01" @default.
- W3174306489 modified "2023-09-27" @default.
- W3174306489 title "Identifying chemotaxis protein‐protein interactions in Epulopiscium sp. Type B using a yeast two hybrid system" @default.
- W3174306489 doi "https://doi.org/10.1096/fasebj.27.1_supplement.1039.3" @default.
- W3174306489 hasPublicationYear "2013" @default.
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