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- W3174339934 abstract "Telomeres, the protective caps of linear chromosomes, have been shown to vary in length between germline and somatic cells of the same organism, leading to the hypothesis that telomeres are lengthened during meiosis. In the filamentous fungus Aspergillus nidulans, which has several different cell types, the telomere lengths in vegetative hyphae and spores are invariant. No study has determined the telomere length of the sexual ascospores. We report the development of a PCR technique to measure the telomere length in A. nidulans, including the meiotic cells. This novel technique that we call “telomere-anchored PCR,” measures telomere length using a fraction of the DNA required for terminal restriction fragment (TRF) Southern analysis. Using this approach, we determined that the A. nidulans ascospore telomere length is identical to telomeres of other cell types from this organism, approximately 110 bp, indicating that a surprisingly strict telomere length regulation exists in the major cell types of A. nidulans. Furthermore, an increase in telomere length is not necessary for meiosis in this organism. When the hyphal telomeres were measured in a telomerase reverse transcriptase knockout strain, decreases in length were readily detected. Thus, this technique can detect telomeres in relatively rare cell types, is sensitive enough to measure them when exceptionally short, and might be utilized in other organisms." @default.
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- W3174339934 date "2012-04-01" @default.
- W3174339934 modified "2023-09-26" @default.
- W3174339934 title "Novel telomere‐anchored PCR approach for studying sexual stage telomeres in Aspergillus nidulans" @default.
- W3174339934 doi "https://doi.org/10.1096/fasebj.26.1_supplement.939.2" @default.
- W3174339934 hasPublicationYear "2012" @default.
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