Matches in SemOpenAlex for { <https://semopenalex.org/work/W3174365744> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W3174365744 abstract "In order to study molecular interactions in the microsomal cytochrome P450 monooxygenase pathway, we have undertaken the development of a NADPH:cytochrome P450 oxidoreductase (CYPOR) with highly enhanced flavin fluorescence while maintaining wild-type (WT) structure and function. Using a bacterial expression system, a soluble human CYPOR lacking the N-terminal 66 residues was produced, comprised of the catalytic FMN-, FAD- and NADPH-binding domains. Using site-directed mutagenesis of our expression plasmid, we have created six variants of human CYPOR; three having substitutions in the FMN-binding domain (Y143F, Y143A, and Y143S) and three in the FAD-binding domain (Y459F, Y459A, and Y459S). All six variants, as well as WT, were expressed and purified to homogeneity. Flavin analysis showed that Y143 variants had slightly decreased FMN content, while Y459 variants had dramatically reduced FAD content, compared to WT. Y143A and Y143S variants exhibited 3.5- and 6.5-fold increases in flavin fluorescence compared to WT, respectively. The Y143S variant retained 85% of WT NADPH:cytochrome c reductase activity, while Y143A was 70% more active than WT. Based on the observed increase in fluorescence and on the retained catalytic efficiency, Y143 variants currently represent the best candidates for development as tools for studying CYPOR interactions with P450s and other microsomal proteins. With proof of principal established, future constructs will incorporate the membrane anchoring residues, for reconstitution of cytochrome P450-mediated activities in vitro and in cellular expression systems (Supported by NIH HL30050 to BSM)." @default.
- W3174365744 created "2021-07-05" @default.
- W3174365744 creator A5005050918 @default.
- W3174365744 creator A5009997661 @default.
- W3174365744 creator A5041418127 @default.
- W3174365744 creator A5062064149 @default.
- W3174365744 creator A5080904988 @default.
- W3174365744 date "2006-03-01" @default.
- W3174365744 modified "2023-09-27" @default.
- W3174365744 title "Engineering a Hyper‐Fluorescent NADPH:Cytochrome P450 Oxidoreductase" @default.
- W3174365744 doi "https://doi.org/10.1096/fasebj.20.4.a474-b" @default.
- W3174365744 hasPublicationYear "2006" @default.
- W3174365744 type Work @default.
- W3174365744 sameAs 3174365744 @default.
- W3174365744 citedByCount "0" @default.
- W3174365744 crossrefType "journal-article" @default.
- W3174365744 hasAuthorship W3174365744A5005050918 @default.
- W3174365744 hasAuthorship W3174365744A5009997661 @default.
- W3174365744 hasAuthorship W3174365744A5041418127 @default.
- W3174365744 hasAuthorship W3174365744A5062064149 @default.
- W3174365744 hasAuthorship W3174365744A5080904988 @default.
- W3174365744 hasConcept C104317684 @default.
- W3174365744 hasConcept C13002179 @default.
- W3174365744 hasConcept C134651460 @default.
- W3174365744 hasConcept C143065580 @default.
- W3174365744 hasConcept C14471203 @default.
- W3174365744 hasConcept C153911025 @default.
- W3174365744 hasConcept C16318435 @default.
- W3174365744 hasConcept C171305022 @default.
- W3174365744 hasConcept C181199279 @default.
- W3174365744 hasConcept C185592680 @default.
- W3174365744 hasConcept C2779268744 @default.
- W3174365744 hasConcept C2780768313 @default.
- W3174365744 hasConcept C28859421 @default.
- W3174365744 hasConcept C29311851 @default.
- W3174365744 hasConcept C33093398 @default.
- W3174365744 hasConcept C526171541 @default.
- W3174365744 hasConcept C55493867 @default.
- W3174365744 hasConcept C86803240 @default.
- W3174365744 hasConcept C87644729 @default.
- W3174365744 hasConceptScore W3174365744C104317684 @default.
- W3174365744 hasConceptScore W3174365744C13002179 @default.
- W3174365744 hasConceptScore W3174365744C134651460 @default.
- W3174365744 hasConceptScore W3174365744C143065580 @default.
- W3174365744 hasConceptScore W3174365744C14471203 @default.
- W3174365744 hasConceptScore W3174365744C153911025 @default.
- W3174365744 hasConceptScore W3174365744C16318435 @default.
- W3174365744 hasConceptScore W3174365744C171305022 @default.
- W3174365744 hasConceptScore W3174365744C181199279 @default.
- W3174365744 hasConceptScore W3174365744C185592680 @default.
- W3174365744 hasConceptScore W3174365744C2779268744 @default.
- W3174365744 hasConceptScore W3174365744C2780768313 @default.
- W3174365744 hasConceptScore W3174365744C28859421 @default.
- W3174365744 hasConceptScore W3174365744C29311851 @default.
- W3174365744 hasConceptScore W3174365744C33093398 @default.
- W3174365744 hasConceptScore W3174365744C526171541 @default.
- W3174365744 hasConceptScore W3174365744C55493867 @default.
- W3174365744 hasConceptScore W3174365744C86803240 @default.
- W3174365744 hasConceptScore W3174365744C87644729 @default.
- W3174365744 hasFunder F4320332161 @default.
- W3174365744 hasIssue "4" @default.
- W3174365744 hasLocation W31743657441 @default.
- W3174365744 hasOpenAccess W3174365744 @default.
- W3174365744 hasPrimaryLocation W31743657441 @default.
- W3174365744 hasRelatedWork W1600563655 @default.
- W3174365744 hasRelatedWork W2011902437 @default.
- W3174365744 hasRelatedWork W2038085261 @default.
- W3174365744 hasRelatedWork W2046376689 @default.
- W3174365744 hasRelatedWork W2068906133 @default.
- W3174365744 hasRelatedWork W2069252522 @default.
- W3174365744 hasRelatedWork W2998667573 @default.
- W3174365744 hasRelatedWork W4245789239 @default.
- W3174365744 hasRelatedWork W967886486 @default.
- W3174365744 hasRelatedWork W2029370404 @default.
- W3174365744 hasVolume "20" @default.
- W3174365744 isParatext "false" @default.
- W3174365744 isRetracted "false" @default.
- W3174365744 magId "3174365744" @default.
- W3174365744 workType "article" @default.