Matches in SemOpenAlex for { <https://semopenalex.org/work/W3174442700> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W3174442700 abstract "Although atherosclerosis manifests itself clinically most commonly in middle-aged and older adults, the origins of the disease, such as vascular plaque formation, can be found in children and adolescents. Understanding monocyte-endothelial cell interactions, a key step in plaque pathogenesis, has been challenging in both adults and children. To overcome this gap, we tested the feasibility of quantifying monocyte-endothelial interaction using an ex-vivo, tissue-engineered microvasculature organ model (VMO) that was originally designed to study mechanisms of cancer metastatic diseases. The VMO mimics the intricacy of a three dimensional vascular bed while allowing for microfluidic pressures to drive monocyte perfusion through the device. PBMCs were isolated from freshly drawn blood (blood donor program). We first established that the most efficacious PBMC concentration for perfusion was 200,000 PBMC/mL. As a proof-of-concept experiment, we tested whether activation of monocytes known to increase vascular “stickiness” could be detected in the VMO. We incubated human PBMCs with either vehicle (control) or 9 ng/mL TNF-afor 24 hours prior to VMO perfusion. PBMCs were stained with CellTracker Green CMFDA fluorescent dye and endothelial cells express mCherry red fluorescent protein. Perfusion was carried out for each condition in replicates of eight. Images were taken at 0, 2, 6, and 24 hours using a Nikon Eclipse Ti90 confocal microscope. TNF-astimulation significantly increased the number of PBMCs that adhered to the vessel walls of our device at 24 hours compared to control (13.6±1.34 vs. 9.25±2.21, p=.0027 t-test). At the 2, 4, and 6 hour time points the difference in adhesion rates between the TNF-astimulated group and control was not significant (3.25±1.89 vs. 5.6±2.30, 6.5±1.29 vs. 9.2±1.48, and 6.5±1.29 vs. 9.2±1.48 respectively). Shown below are representative images of the vascular micro-organ device taken at 24 hours following PBMC perfusion. These preliminary results indicate that our VMO model can provide insight regarding the inflammatory and pathogenic role of human monocyte dysregulation and may be useful in detecting environmental and lifestyle factors that could ameliorate the development of atherosclerosis early in life. Support or Funding Information UCI Convergence Research Program This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal." @default.
- W3174442700 created "2021-07-05" @default.
- W3174442700 creator A5024191718 @default.
- W3174442700 creator A5033468427 @default.
- W3174442700 creator A5050737670 @default.
- W3174442700 creator A5066152386 @default.
- W3174442700 creator A5090080497 @default.
- W3174442700 date "2019-04-01" @default.
- W3174442700 modified "2023-10-16" @default.
- W3174442700 title "A novel in vitro model of the microvasculature to study monocyte activation" @default.
- W3174442700 doi "https://doi.org/10.1096/fasebj.2019.33.1_supplement.496.65" @default.
- W3174442700 hasPublicationYear "2019" @default.
- W3174442700 type Work @default.
- W3174442700 sameAs 3174442700 @default.
- W3174442700 citedByCount "0" @default.
- W3174442700 crossrefType "journal-article" @default.
- W3174442700 hasAuthorship W3174442700A5024191718 @default.
- W3174442700 hasAuthorship W3174442700A5033468427 @default.
- W3174442700 hasAuthorship W3174442700A5050737670 @default.
- W3174442700 hasAuthorship W3174442700A5066152386 @default.
- W3174442700 hasAuthorship W3174442700A5090080497 @default.
- W3174442700 hasConcept C126322002 @default.
- W3174442700 hasConcept C136009344 @default.
- W3174442700 hasConcept C137061746 @default.
- W3174442700 hasConcept C142724271 @default.
- W3174442700 hasConcept C146957229 @default.
- W3174442700 hasConcept C150903083 @default.
- W3174442700 hasConcept C185592680 @default.
- W3174442700 hasConcept C202751555 @default.
- W3174442700 hasConcept C203014093 @default.
- W3174442700 hasConcept C207001950 @default.
- W3174442700 hasConcept C2524010 @default.
- W3174442700 hasConcept C26291073 @default.
- W3174442700 hasConcept C2776992346 @default.
- W3174442700 hasConcept C2779178360 @default.
- W3174442700 hasConcept C2780942790 @default.
- W3174442700 hasConcept C2781184567 @default.
- W3174442700 hasConcept C33923547 @default.
- W3174442700 hasConcept C55493867 @default.
- W3174442700 hasConcept C71924100 @default.
- W3174442700 hasConcept C86803240 @default.
- W3174442700 hasConcept C95444343 @default.
- W3174442700 hasConceptScore W3174442700C126322002 @default.
- W3174442700 hasConceptScore W3174442700C136009344 @default.
- W3174442700 hasConceptScore W3174442700C137061746 @default.
- W3174442700 hasConceptScore W3174442700C142724271 @default.
- W3174442700 hasConceptScore W3174442700C146957229 @default.
- W3174442700 hasConceptScore W3174442700C150903083 @default.
- W3174442700 hasConceptScore W3174442700C185592680 @default.
- W3174442700 hasConceptScore W3174442700C202751555 @default.
- W3174442700 hasConceptScore W3174442700C203014093 @default.
- W3174442700 hasConceptScore W3174442700C207001950 @default.
- W3174442700 hasConceptScore W3174442700C2524010 @default.
- W3174442700 hasConceptScore W3174442700C26291073 @default.
- W3174442700 hasConceptScore W3174442700C2776992346 @default.
- W3174442700 hasConceptScore W3174442700C2779178360 @default.
- W3174442700 hasConceptScore W3174442700C2780942790 @default.
- W3174442700 hasConceptScore W3174442700C2781184567 @default.
- W3174442700 hasConceptScore W3174442700C33923547 @default.
- W3174442700 hasConceptScore W3174442700C55493867 @default.
- W3174442700 hasConceptScore W3174442700C71924100 @default.
- W3174442700 hasConceptScore W3174442700C86803240 @default.
- W3174442700 hasConceptScore W3174442700C95444343 @default.
- W3174442700 hasIssue "S1" @default.
- W3174442700 hasLocation W31744427001 @default.
- W3174442700 hasOpenAccess W3174442700 @default.
- W3174442700 hasPrimaryLocation W31744427001 @default.
- W3174442700 hasRelatedWork W1975528830 @default.
- W3174442700 hasRelatedWork W2025993790 @default.
- W3174442700 hasRelatedWork W2042957539 @default.
- W3174442700 hasRelatedWork W2060285676 @default.
- W3174442700 hasRelatedWork W2074766836 @default.
- W3174442700 hasRelatedWork W2080971289 @default.
- W3174442700 hasRelatedWork W2138422753 @default.
- W3174442700 hasRelatedWork W2412272801 @default.
- W3174442700 hasRelatedWork W4214725470 @default.
- W3174442700 hasRelatedWork W4294684307 @default.
- W3174442700 hasVolume "33" @default.
- W3174442700 isParatext "false" @default.
- W3174442700 isRetracted "false" @default.
- W3174442700 magId "3174442700" @default.
- W3174442700 workType "article" @default.