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- W3174443767 abstract "The development of high-throughput technologies has revealed pervasive transcription in all genomes that have been investigated so far. This has uncovered a highly interleaved transcriptome organization involving thousands of overlapping coding and non-coding RNA isoforms that challenge our traditional definitions of genes and functional regions of the genome. In this chapter, we discuss the application of an improved Transcript Isoform Sequencing approach (TIF-Seq2) able to concurrently determine the start and end sites of individual RNA molecules. We exemplify its use for the investigation of the human transcriptome and show how it is especially well suited to discriminate between overlapping molecules and accurately define their boundaries." @default.
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- W3174443767 date "2021-01-01" @default.
- W3174443767 modified "2023-10-09" @default.
- W3174443767 title "Using TIF-Seq2 to investigate association between 5´ and 3´mRNA ends" @default.
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- W3174443767 doi "https://doi.org/10.1016/bs.mie.2021.03.017" @default.
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