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- W3174978342 abstract "Abstract The most successful genetically encoded calcium indicators (GECIs) employ an intensity or intensiometric readout. Despite a large calcium-dependent change in fluorescence intensity, the quantification of calcium concentrations with GECIs is problematic, which is further complicated by the sensitivity of all GECIs to changes in the pH in the biological range. Here, we report on a novel sensing strategy in which a conformational change directly modifies the fluorescence quantum yield and fluorescence lifetime of a circular permutated turquoise fluorescent protein. The fluorescence lifetime is an absolute parameter that enables straightforward quantification, eliminating intensity-related artifacts. A new engineering strategy that optimizes lifetime contrast led to a biosensor that shows a 3-fold change in the calcium-dependent quantum yield and a fluorescence lifetime change of 1.3 ns. Additionally, the response of the calcium sensor is insensitive to pH between 6.2-9. As a result, the turquoise GECI enables robust measurements of intracellular calcium concentrations by fluorescence lifetime imaging. We demonstrate quantitative imaging of calcium concentration with the turquoise GECI in single endothelial cells and human-derived organoids. Abstract Figure" @default.
- W3174978342 created "2021-07-05" @default.
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- W3174978342 date "2021-06-21" @default.
- W3174978342 modified "2023-10-16" @default.
- W3174978342 title "A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium" @default.
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- W3174978342 doi "https://doi.org/10.1101/2021.06.21.449214" @default.
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