FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W3175122010> ?p ?o ?g. }

• W3175122010 endingPage "1688" @default.
• W3175122010 startingPage "1682" @default.
• W3175122010 abstract "Canine parvovirus (CPV) is one of the most common viral infections in dogs, causing acute hemorrhagic gastroenteritis and high mortality. Vaccination effectively prevents CPV infection. However, the currently available CPV vaccines have concerns such as maternal immunity interference, shedding of virus vaccine, and false-positive result based on polymerase chain reaction after vaccination. A subunit vaccine can overcome these problems. This study aimed to express the recombinant 35 kDa fragment of the VP2 protein (consisting of epitopes 1-7) and the recombinant full-length VP2 protein (consisting of epitopes 1-10) and to study the ability of these two recombinant proteins to react with rabbit anti-CPV polyclonal antibodies.The full length and 35 kDa fragment of VP2 gene of CPV were cloned into the pBAD202 Directional TOPO™ expression vector and expressed in E. coli. The recombinant full-length and the recombinant 35 kDa fragment proteins of VP2 were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.The recombinant full-length and the recombinant 35 kDa fragment VP2 genes were successfully cloned and expressed. The optimum concentrations of arabinose and induction time for the recombinant full-length and the recombinant 35 kDa fragment VP2 proteins were 0.2% for 6 h and 0.02% for 6 h, respectively. The recombinant full-length and the recombinant 35 kDa fragment VP2 protein molecular weights were approximately 81 and 51 kDa, respectively. The recombinant full-length and the recombinant 35 kDa fragment VP2 proteins specifically interacted with rabbit anti-CPV polyclonal antibodies.These results suggest that the recombinant 35 kDa fragment and the recombinant full-length VP2 proteins may be useful in developing a CPV diagnostic test or vaccine." @default.
• W3175122010 created "2021-07-05" @default.
• W3175122010 creator A5001849276 @default.
• W3175122010 creator A5010705486 @default.
• W3175122010 creator A5015914840 @default.
• W3175122010 creator A5018716703 @default.
• W3175122010 creator A5041927551 @default.
• W3175122010 creator A5054003509 @default.
• W3175122010 creator A5068345782 @default.
• W3175122010 creator A5069403522 @default.
• W3175122010 creator A5088508238 @default.
• W3175122010 date "2021-06-29" @default.
• W3175122010 modified "2023-10-16" @default.
• W3175122010 title "Expression of recombinant 35 kDa fragment of VP2 protein of canine parvovirus using Escherichia coli expression system" @default.
• W3175122010 cites W1991016406 @default.
• W3175122010 cites W2016094282 @default.
• W3175122010 cites W2043114593 @default.
• W3175122010 cites W2044310913 @default.
• W3175122010 cites W2046182783 @default.
• W3175122010 cites W2074312008 @default.
• W3175122010 cites W2083830261 @default.
• W3175122010 cites W2095417833 @default.
• W3175122010 cites W2097446945 @default.
• W3175122010 cites W2115193182 @default.
• W3175122010 cites W2118812359 @default.
• W3175122010 cites W2148462715 @default.
• W3175122010 cites W2208938394 @default.
• W3175122010 cites W2295233517 @default.
• W3175122010 cites W2339647820 @default.
• W3175122010 cites W2464654989 @default.
• W3175122010 cites W2472268813 @default.
• W3175122010 cites W2473988462 @default.
• W3175122010 cites W2496442204 @default.
• W3175122010 cites W2728974750 @default.
• W3175122010 cites W2912033430 @default.
• W3175122010 cites W2977585878 @default.
• W3175122010 cites W3006420901 @default.
• W3175122010 cites W3011161229 @default.
• W3175122010 cites W4244804637 @default.
• W3175122010 doi "https://doi.org/10.14202/vetworld.2021.1682-1688" @default.
• W3175122010 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8304427" @default.
• W3175122010 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34316219" @default.
• W3175122010 hasPublicationYear "2021" @default.
• W3175122010 type Work @default.
• W3175122010 sameAs 3175122010 @default.
• W3175122010 citedByCount "0" @default.
• W3175122010 crossrefType "journal-article" @default.
• W3175122010 hasAuthorship W3175122010A5001849276 @default.
• W3175122010 hasAuthorship W3175122010A5010705486 @default.
• W3175122010 hasAuthorship W3175122010A5015914840 @default.
• W3175122010 hasAuthorship W3175122010A5018716703 @default.
• W3175122010 hasAuthorship W3175122010A5041927551 @default.
• W3175122010 hasAuthorship W3175122010A5054003509 @default.
• W3175122010 hasAuthorship W3175122010A5068345782 @default.
• W3175122010 hasAuthorship W3175122010A5069403522 @default.
• W3175122010 hasAuthorship W3175122010A5088508238 @default.
• W3175122010 hasBestOaLocation W31751220101 @default.
• W3175122010 hasConcept C104317684 @default.
• W3175122010 hasConcept C123894998 @default.
• W3175122010 hasConcept C136071829 @default.
• W3175122010 hasConcept C153911025 @default.
• W3175122010 hasConcept C159047783 @default.
• W3175122010 hasConcept C159654299 @default.
• W3175122010 hasConcept C195616568 @default.
• W3175122010 hasConcept C203014093 @default.
• W3175122010 hasConcept C2522874641 @default.
• W3175122010 hasConcept C2779630601 @default.
• W3175122010 hasConcept C2780339557 @default.
• W3175122010 hasConcept C40767141 @default.
• W3175122010 hasConcept C547475151 @default.
• W3175122010 hasConcept C55493867 @default.
• W3175122010 hasConcept C86803240 @default.
• W3175122010 hasConcept C956191 @default.
• W3175122010 hasConceptScore W3175122010C104317684 @default.
• W3175122010 hasConceptScore W3175122010C123894998 @default.
• W3175122010 hasConceptScore W3175122010C136071829 @default.
• W3175122010 hasConceptScore W3175122010C153911025 @default.
• W3175122010 hasConceptScore W3175122010C159047783 @default.
• W3175122010 hasConceptScore W3175122010C159654299 @default.
• W3175122010 hasConceptScore W3175122010C195616568 @default.
• W3175122010 hasConceptScore W3175122010C203014093 @default.
• W3175122010 hasConceptScore W3175122010C2522874641 @default.
• W3175122010 hasConceptScore W3175122010C2779630601 @default.
• W3175122010 hasConceptScore W3175122010C2780339557 @default.
• W3175122010 hasConceptScore W3175122010C40767141 @default.
• W3175122010 hasConceptScore W3175122010C547475151 @default.
• W3175122010 hasConceptScore W3175122010C55493867 @default.
• W3175122010 hasConceptScore W3175122010C86803240 @default.
• W3175122010 hasConceptScore W3175122010C956191 @default.
• W3175122010 hasFunder F4320329432 @default.
• W3175122010 hasLocation W31751220101 @default.
• W3175122010 hasLocation W31751220102 @default.
• W3175122010 hasLocation W31751220103 @default.
• W3175122010 hasLocation W31751220104 @default.
• W3175122010 hasOpenAccess W3175122010 @default.
• W3175122010 hasPrimaryLocation W31751220101 @default.
• W3175122010 hasRelatedWork W2039736738 @default.
• W3175122010 hasRelatedWork W2068999859 @default.

• next