Matches in SemOpenAlex for { <https://semopenalex.org/work/W3175146581> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W3175146581 abstract "Intracellular calcium (Ca2+) is involved in distinct cellular functions through global and local signaling in various subcellular compartments. To get better insight if the Ca2+ signals are independently regulated in subcellular compartments, we targeted genetically encoded Ca2+ indicator, D3cpv, into three different subcellular regions and used laser scanning confocal microscopy to study how Ca2+ signals are triggered in the plasma membrane (PM), cytoplasm and nucleus in cultured rat pulmonary artery smooth muscle cells (PASMCs). The in situ Kd for Ca2+ of D3cpv was comparable in the cytoplasm and nucleoplasm, but it was slightly higher in the PM. Application of IP3 or Endothelin-1 to PASMCs caused rapid increase of fluorescence emission ratio in all three regions. The increase of cytoplasmic/PM Ca2+ preceded the nucleoplasmic Ca2+ and the response was more sustained in the nucleus. Platelet derived growth factor (PDGF) generated variable Ca2+ signals with Ca2+ increase occurred first in the cytoplasm followed by the nucleoplasm in some cells, while an early Ca2+ response in the nucleus was observed in the others. Similarly, the increase in PM Ca2+ was slower than that in the nucleoplasm in some PDGF treated cells. These data suggest a differential regulation of Ca2+ in the cellular compartments that may be essential for the independent regulation of contraction, cell signaling and gene expression in PASMCs." @default.
- W3175146581 created "2021-07-05" @default.
- W3175146581 creator A5007999997 @default.
- W3175146581 creator A5024758785 @default.
- W3175146581 creator A5028289577 @default.
- W3175146581 date "2013-04-01" @default.
- W3175146581 modified "2023-10-14" @default.
- W3175146581 title "Probing differential subcellular calcium dynamics using targeted fluorescence calcium biosensors in rat pulmonary artery smooth muscle cells" @default.
- W3175146581 doi "https://doi.org/10.1096/fasebj.27.1_supplement.923.18" @default.
- W3175146581 hasPublicationYear "2013" @default.
- W3175146581 type Work @default.
- W3175146581 sameAs 3175146581 @default.
- W3175146581 citedByCount "0" @default.
- W3175146581 crossrefType "journal-article" @default.
- W3175146581 hasAuthorship W3175146581A5007999997 @default.
- W3175146581 hasAuthorship W3175146581A5024758785 @default.
- W3175146581 hasAuthorship W3175146581A5028289577 @default.
- W3175146581 hasConcept C112952253 @default.
- W3175146581 hasConcept C12554922 @default.
- W3175146581 hasConcept C178790620 @default.
- W3175146581 hasConcept C181199279 @default.
- W3175146581 hasConcept C183873130 @default.
- W3175146581 hasConcept C185592680 @default.
- W3175146581 hasConcept C190062978 @default.
- W3175146581 hasConcept C2776879804 @default.
- W3175146581 hasConcept C2780723820 @default.
- W3175146581 hasConcept C519063684 @default.
- W3175146581 hasConcept C55493867 @default.
- W3175146581 hasConcept C86803240 @default.
- W3175146581 hasConcept C95444343 @default.
- W3175146581 hasConcept C98539663 @default.
- W3175146581 hasConceptScore W3175146581C112952253 @default.
- W3175146581 hasConceptScore W3175146581C12554922 @default.
- W3175146581 hasConceptScore W3175146581C178790620 @default.
- W3175146581 hasConceptScore W3175146581C181199279 @default.
- W3175146581 hasConceptScore W3175146581C183873130 @default.
- W3175146581 hasConceptScore W3175146581C185592680 @default.
- W3175146581 hasConceptScore W3175146581C190062978 @default.
- W3175146581 hasConceptScore W3175146581C2776879804 @default.
- W3175146581 hasConceptScore W3175146581C2780723820 @default.
- W3175146581 hasConceptScore W3175146581C519063684 @default.
- W3175146581 hasConceptScore W3175146581C55493867 @default.
- W3175146581 hasConceptScore W3175146581C86803240 @default.
- W3175146581 hasConceptScore W3175146581C95444343 @default.
- W3175146581 hasConceptScore W3175146581C98539663 @default.
- W3175146581 hasIssue "S1" @default.
- W3175146581 hasLocation W31751465811 @default.
- W3175146581 hasOpenAccess W3175146581 @default.
- W3175146581 hasPrimaryLocation W31751465811 @default.
- W3175146581 hasRelatedWork W107716262 @default.
- W3175146581 hasRelatedWork W2024324386 @default.
- W3175146581 hasRelatedWork W2024489118 @default.
- W3175146581 hasRelatedWork W2049551611 @default.
- W3175146581 hasRelatedWork W2064440555 @default.
- W3175146581 hasRelatedWork W2161063591 @default.
- W3175146581 hasRelatedWork W2266370831 @default.
- W3175146581 hasRelatedWork W2395626159 @default.
- W3175146581 hasRelatedWork W4233786165 @default.
- W3175146581 hasRelatedWork W96789612 @default.
- W3175146581 hasVolume "27" @default.
- W3175146581 isParatext "false" @default.
- W3175146581 isRetracted "false" @default.
- W3175146581 magId "3175146581" @default.
- W3175146581 workType "article" @default.