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• W3175291267 abstract "Abstract In female mammals, the proliferation, apoptosis, and estradiol-17β (E2) secretion of granulosa cells (GCs) have come to decide the fate of follicles. DNA methylation and RSPO2 gene of Wnt signaling pathway have been reported to involve in the survival of GCs and follicular development. However, the molecular mechanisms for how DNA methylation regulates the expression of RSPO2 and participates in the follicular development are not clear. In this study, we found that the mRNA and protein levels of RSPO2 significantly increased during follicular development, but the DNA methylation level of RSPO2 promoter decreased gradually. Inhibition of DNA methylation or DNMT1 knockdown could decrease the methylation level of CpG island (CGI) in RSPO2 promoter and upregulate the expression level of RSPO2 in porcine GCs. The hypomethylation of −758/−749 and −563/−553 regions in RSPO2 promoter facilitated the occupancy of transcription factor E2F1 and promoted the transcriptional activity of RSPO2 . Moreover, RSPO2 promoted the proliferation of GCs with increasing the expression level of PCNA , CDK1 , and CCND1 and promoted the E2 secretion of GCs with increasing the expression level of CYP19A1 and HSD17B1 and inhibited the apoptosis of GCs with decreasing the expression level of Caspase3 , cleaved Caspase3 , cleaved Caspase8 , cleaved Caspase9 , cleaved PARP , and BAX . In addition, RSPO2 knockdown promoted the apoptosis of GCs, blocked the development of follicles, and delayed the onset of puberty with decreasing the expression level of Wnt signaling pathway-related genes ( LGR4 and CTNNB1 ) in vivo. Taken together, the hypomethylation of −758/−749 and −563/−553 regions in RSPO2 promoter facilitated the occupancy of E2F1 and enhanced the transcription of RSPO2 , which further promoted the proliferation and E2 secretion of GCs, inhibited the apoptosis of GCs, and ultimately ameliorated the development of follicles through Wnt signaling pathway. This study will provide useful information for further exploration on DNA-methylation-mediated RSPO2 pathway during follicular development." @default.
• W3175291267 created "2021-07-05" @default.
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• W3175291267 date "2021-06-26" @default.
• W3175291267 modified "2023-10-09" @default.
• W3175291267 title "DNA methylation mediated RSPO2 to promote follicular development in mammals" @default.
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• W3175291267 doi "https://doi.org/10.1038/s41419-021-03941-z" @default.
• W3175291267 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8236063" @default.
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• W3175291267 hasPublicationYear "2021" @default.
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