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• W3175599594 abstract "Abstract The p47 immunity-related GTPase (IRG) Irgb6 plays a pioneering role in host defense against Toxoplasma gondii infection. It is recruited to the parasitophorous vacuole membrane (PVM) formed by T. gondii and disrupts it. Despite the importance of this process, the molecular mechanisms accounting for PVM recognition by Irgb6 remain elusive due to lack of structural information on Irgb6. Here we report the crystal structures of mouse Irgb6 in the GTP-bound and nucleotide-free forms. Irgb6 exhibits a similar overall architecture to other IRGs in which GTP-binding induces conformational changes in both the dimerization interface and the membrane-binding interface. The membrane-binding interface of Irgb6 assumes a unique conformation, composed of N- and C-terminal helical regions forming a phospholipid binding site. In silico docking of phospholipids further revealed membrane binding residues that were validated through mutagenesis and cell-based assays. Collectively, these data demonstrate a novel structural basis for Irgb6 to recognize T. gondii PVM in a manner distinct from other IRGs. Summary Upon Toxoplasma gondii infection, Irgb6 is recruited to the parasitophorous vacuole membrane (PVM) where it disrupts it. We solved the atomic structures of Irgb6 in two distinct nucleotide states, revealing a unique PVM binding interface sensitive to the GTPase cycling." @default.
• W3175599594 created "2021-07-05" @default.
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• W3175599594 date "2021-07-01" @default.
• W3175599594 modified "2023-09-30" @default.
• W3175599594 title "Structural basis of membrane recognition of Toxoplasma gondii vacuole by Irgb6" @default.
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• W3175599594 doi "https://doi.org/10.1101/2021.07.01.450801" @default.
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