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• W3175790634 endingPage "31724" @default.
• W3175790634 startingPage "31710" @default.
• W3175790634 abstract "In this study, we have designed a three-fluorophore-labeled Y-shaped DNAzyme with a high catalytic cleavage activity and a three-dimensional (3D) MOF-MoS2NB (metal–organic framework fused with molybdenum disulfide nanobox), which was synthesized as an efficient quencher of the fluorescent biosensor. The synthesized porous 3D MOF-MoS2NBs and Y-shaped DNAzyme exhibited a good analytical response toward the simultaneous multiple detections of Hg2+, Ni2+, and Ag+ ions over the other coexisting metal ions. More specifically, the three kinds of enzyme aptamer and substrate aptamer (SA) were hybridized and annealed to form the Y-shaped DNAzyme structure and labeled with three different fluorophores such as FAM, TAMRA, and ROX over the 3′-end of SA. When the targets were induced, the DNAzyme was triggered to cleave the fluorophore-labeled SAs. Then, the cleaved SAs (FAM-SA, TAMRA-SA, and ROX-SA) were adsorbed on the 3D MOF-MoS2NB surface to quench the fluorescence signal due to a noncovalent interaction (van der Waals and π–π stacking interaction), which transmuted the fluorescence on-state to off-state. As a result, the fluorescence assay confiscated the high selectivity and sensitivity for the target analytes of Hg2+, Ni2+, and Ag+ ions achieved for the detection limits of 0.11 nM, 7.8 μM, and 0.25 nM, respectively. Accordingly, the sensitivity of the developed sensor was explored with a better lower detection limit than the previously reported biosensors. The utility of the designed Y-shaped DNAzyme may find a broad field of application in real water sample analysis with interfering contaminants." @default.
• W3175790634 created "2021-07-05" @default.
• W3175790634 creator A5031054365 @default.
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• W3175790634 date "2021-07-02" @default.
• W3175790634 modified "2023-10-10" @default.
• W3175790634 title "High Catalytic Activity of Fluorophore-Labeled Y-Shaped DNAzyme/3D MOF-MoS₂NBs as a Versatile Biosensing Platform for the Simultaneous Detection of Hg²⁺, Ni²⁺, and Ag⁺ Ions" @default.
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• W3175790634 doi "https://doi.org/10.1021/acsami.1c07086" @default.
• W3175790634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34213303" @default.
• W3175790634 hasPublicationYear "2021" @default.
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