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- W317586793 abstract "This chapter aims to describe methods for the preparation of cell extracts and the purification, and assay of holoenzyme activity. The Brassica pol I holoenzyme purification is described in detail in this chapter. Alterations in this protocol relevant to the purification of pol I holoenzyme activity from cultured Xenopus cells is also explained. It explores that transcription of ribosomal RNA by RNA polymerase I (pol) is a ratelimiting process for ribosome production and hence for protein synthesis, cell growth, and cell proliferation. RNA polymerase I can be purified in a “holoenzyme” form capable of promoter recognition and accurate transcription initiation in vitro. Initial evidence for a pol I holoenzyme came from biochemical studies in plants using broccoli as an inexpensive and abundant source of proliferating cells. Pol I holoenzymes can also be purified from vertebrates. This chapter presents the methods for the purification and transcriptional analysis of RNA polymerase I holoenzyme from broccoli and cultured Xenopus cells." @default.
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- W317586793 date "2003-01-01" @default.
- W317586793 modified "2023-10-18" @default.
- W317586793 title "Purification and Transcriptional Analysis of RNA Polymerase I Holoenzymes from Broccoli (Brassica oleracea) and Frog (Xenopus laevis)" @default.
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- W317586793 doi "https://doi.org/10.1016/s0076-6879(03)70011-1" @default.
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