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- W3175890308 abstract "Glucagon stimulates the vesicle trafficking of aquaporin-8 (AQP8) water channels to the canalicular membrane of rat hepatocytes, a mechanism thought to be involved in glucagon-induced bile secretion. Our aim was to study whether glucagon is also able to stimulate the gene expression of hepatocyte AQP8. Glucagon was administered to rats at 0.2 mg/100g body wt ip for 8, 16, and 36 h. By immunoblotting, we found that 34 kDa AQP8 was increased by 79% and 107% in total liver membranes at 16 and 36 h. Hepatocyte AQP9 protein expression remained unaltered. AQP8 mRNA expression, assessed by real time PCR, was not modified over time. Immunoblotting studies in primary cultured rat hepatocytes showed that glucagon increased the total amount of AQP8 (e.g. 80% with 1μM glucagon) and confocal immunofluorescence showed a predominant AQP8 cell surface localization. The RNA polymerase II inhibitor actinomycin D did not prevent glucagon effect supporting the nontranscriptional upregulation of hepatocyte AQP8. Glucagon effect on AQP8 was mimicked by dibutyryl cAMP and supressed by the protein kinase A (PKA) inhibitors, H-89 or PKI. In conclusion, our data suggest that glucagon induces the posttranscriptional gene expression of hepatocyte AQP8 via a cAMP-dependent PKA pathway, a process likely relevant to the long-term modulation of bile secretion. Supported by Grant PICT 31670 from ANPCyT" @default.
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- W3175890308 date "2008-03-01" @default.
- W3175890308 modified "2023-10-14" @default.
- W3175890308 title "Glucagon induces the protein expression of rat hepatocyte aquaporin‐8." @default.
- W3175890308 doi "https://doi.org/10.1096/fasebj.22.1_supplement.1190.4" @default.
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