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- W3175942228 endingPage "100912" @default.
- W3175942228 startingPage "100912" @default.
- W3175942228 abstract "The translesion synthesis (TLS) DNA polymerases Rev1 and Polζ function together in DNA lesion bypass during DNA replication, acting as nucleotide inserter and extender polymerases, respectively. While the structural characterization of the Saccharomyces cerevisiae Polζ in its DNA-bound state has illuminated how this enzyme synthesizes DNA, a mechanistic understanding of TLS also requires probing conformational changes associated with DNA- and Rev1 binding. Here, we used single-particle cryo-electron microscopy to determine the structure of the apo Polζ holoenzyme. We show that compared with its DNA-bound state, apo Polζ displays enhanced flexibility that correlates with concerted motions associated with expansion of the Polζ DNA-binding channel upon DNA binding. We also identified a lysine residue that obstructs the DNA-binding channel in apo Polζ, suggesting a gating mechanism. The Polζ subunit Rev7 is a hub protein that directly binds Rev1 and is a component of several other protein complexes such as the shieldin DNA double-strand break repair complex. We analyzed the molecular interactions of budding yeast Rev7 in the context of Polζ and those of human Rev7 in the context of shieldin using a crystal structure of Rev7 bound to a fragment of the shieldin-3 protein. Overall, our study provides new insights into Polζ mechanism of action and the manner in which Rev7 recognizes partner proteins." @default.
- W3175942228 created "2021-07-05" @default.
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- W3175942228 date "2021-08-01" @default.
- W3175942228 modified "2023-09-30" @default.
- W3175942228 title "Cryo-EM reveals conformational flexibility in apo DNA polymerase ζ" @default.
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- W3175942228 cites W1971658781 @default.
- W3175942228 cites W1987586798 @default.
- W3175942228 cites W1990785396 @default.
- W3175942228 cites W1996368299 @default.
- W3175942228 cites W1997029264 @default.
- W3175942228 cites W2015556811 @default.
- W3175942228 cites W2018179063 @default.
- W3175942228 cites W2024703739 @default.
- W3175942228 cites W2032761377 @default.
- W3175942228 cites W2034484189 @default.
- W3175942228 cites W2052965880 @default.
- W3175942228 cites W2057218806 @default.
- W3175942228 cites W2062487397 @default.
- W3175942228 cites W2063079466 @default.
- W3175942228 cites W2075824676 @default.
- W3175942228 cites W2090079351 @default.
- W3175942228 cites W2092869952 @default.
- W3175942228 cites W2094302275 @default.
- W3175942228 cites W2094549019 @default.
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- W3175942228 cites W2104130324 @default.
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- W3175942228 cites W2111616806 @default.
- W3175942228 cites W2112078820 @default.
- W3175942228 cites W2120269671 @default.
- W3175942228 cites W2121671527 @default.
- W3175942228 cites W2124026197 @default.
- W3175942228 cites W2125982181 @default.
- W3175942228 cites W2132629607 @default.
- W3175942228 cites W2133723813 @default.
- W3175942228 cites W2135333576 @default.
- W3175942228 cites W2142161929 @default.
- W3175942228 cites W2142513910 @default.
- W3175942228 cites W2143064529 @default.
- W3175942228 cites W2144081223 @default.
- W3175942228 cites W2156431807 @default.
- W3175942228 cites W2167668429 @default.
- W3175942228 cites W2167960686 @default.
- W3175942228 cites W2180229411 @default.
- W3175942228 cites W2191960775 @default.
- W3175942228 cites W2587625522 @default.
- W3175942228 cites W2728385286 @default.
- W3175942228 cites W2734356283 @default.
- W3175942228 cites W2747521922 @default.
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- W3175942228 cites W2884164644 @default.
- W3175942228 cites W2885651273 @default.
- W3175942228 cites W2906661501 @default.
- W3175942228 cites W2948035094 @default.
- W3175942228 cites W2951611668 @default.
- W3175942228 cites W2951628012 @default.
- W3175942228 cites W2967259419 @default.
- W3175942228 cites W2967411401 @default.
- W3175942228 cites W2967431278 @default.
- W3175942228 cites W2977632608 @default.
- W3175942228 cites W2993834615 @default.
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- W3175942228 doi "https://doi.org/10.1016/j.jbc.2021.100912" @default.
- W3175942228 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8319531" @default.
- W3175942228 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34174285" @default.
- W3175942228 hasPublicationYear "2021" @default.
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