Matches in SemOpenAlex for { <https://semopenalex.org/work/W3175995000> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W3175995000 abstract "ZnT2 plays an important role in zinc (Zn) secretion from the mammary gland and it is regulated by prolactin (PRL) during lactation. Herein, the objective was to explore the mechanisms through which PRL regulates ZnT2 expression in mammary cells. We determined that PRL increases ZnT2 mRNA level in cultured mammary epithelial cells (HC11) which was inhibited by pre-treatment with the Jak2 inhibitor AG490. A fragment of the ZnT2 promoter was subcloned into a luciferase reporter vector and transfected into HC11 cells. The activity of the ZnT2 promoter was increased by PRL treatment. Cells co-transfected with a dominant-negative STAT5 abrogated this effect implicating the Jak2/STAT5 signaling pathway in the transcriptional regulation of ZnT2. Two putative consensus STAT5 binding sequences (GAS1 and GAS2) were identified in the ZnT2 promoter. Gel shift assays indicated that PRL induced STAT5 binding to the proximal GAS2 element with greater efficiency as compared with the distal GAS1 element. Mutagenesis analysis of these two GAS elements suggests that they synergistically modulate ZnT2 transcription. Finally, chromosome immunoprecipition assays confirmed that PRL activates STAT5 binding to the ZnT2 promoter in vivo. Our results indicate that PRL regulates ZnT2 transcription through activation of the Jak2/STAT5 signaling pathway coordinating the regulation of mammary Zn secretion processes." @default.
- W3175995000 created "2021-07-05" @default.
- W3175995000 creator A5038541716 @default.
- W3175995000 creator A5053198596 @default.
- W3175995000 creator A5060725632 @default.
- W3175995000 creator A5075344451 @default.
- W3175995000 date "2009-04-01" @default.
- W3175995000 modified "2023-10-16" @default.
- W3175995000 title "Prolactin regulates ZnT2 transcription through the JAK2‐STAT5 signaling pathway" @default.
- W3175995000 doi "https://doi.org/10.1096/fasebj.23.1_supplement.724.7" @default.
- W3175995000 hasPublicationYear "2009" @default.
- W3175995000 type Work @default.
- W3175995000 sameAs 3175995000 @default.
- W3175995000 citedByCount "0" @default.
- W3175995000 crossrefType "journal-article" @default.
- W3175995000 hasAuthorship W3175995000A5038541716 @default.
- W3175995000 hasAuthorship W3175995000A5053198596 @default.
- W3175995000 hasAuthorship W3175995000A5060725632 @default.
- W3175995000 hasAuthorship W3175995000A5075344451 @default.
- W3175995000 hasConcept C101762097 @default.
- W3175995000 hasConcept C104317684 @default.
- W3175995000 hasConcept C111335760 @default.
- W3175995000 hasConcept C138885662 @default.
- W3175995000 hasConcept C150194340 @default.
- W3175995000 hasConcept C153911025 @default.
- W3175995000 hasConcept C161733203 @default.
- W3175995000 hasConcept C179926584 @default.
- W3175995000 hasConcept C185592680 @default.
- W3175995000 hasConcept C2779064019 @default.
- W3175995000 hasConcept C2779391668 @default.
- W3175995000 hasConcept C41895202 @default.
- W3175995000 hasConcept C54009773 @default.
- W3175995000 hasConcept C55493867 @default.
- W3175995000 hasConcept C62478195 @default.
- W3175995000 hasConcept C71315377 @default.
- W3175995000 hasConcept C86339819 @default.
- W3175995000 hasConcept C86803240 @default.
- W3175995000 hasConcept C95444343 @default.
- W3175995000 hasConceptScore W3175995000C101762097 @default.
- W3175995000 hasConceptScore W3175995000C104317684 @default.
- W3175995000 hasConceptScore W3175995000C111335760 @default.
- W3175995000 hasConceptScore W3175995000C138885662 @default.
- W3175995000 hasConceptScore W3175995000C150194340 @default.
- W3175995000 hasConceptScore W3175995000C153911025 @default.
- W3175995000 hasConceptScore W3175995000C161733203 @default.
- W3175995000 hasConceptScore W3175995000C179926584 @default.
- W3175995000 hasConceptScore W3175995000C185592680 @default.
- W3175995000 hasConceptScore W3175995000C2779064019 @default.
- W3175995000 hasConceptScore W3175995000C2779391668 @default.
- W3175995000 hasConceptScore W3175995000C41895202 @default.
- W3175995000 hasConceptScore W3175995000C54009773 @default.
- W3175995000 hasConceptScore W3175995000C55493867 @default.
- W3175995000 hasConceptScore W3175995000C62478195 @default.
- W3175995000 hasConceptScore W3175995000C71315377 @default.
- W3175995000 hasConceptScore W3175995000C86339819 @default.
- W3175995000 hasConceptScore W3175995000C86803240 @default.
- W3175995000 hasConceptScore W3175995000C95444343 @default.
- W3175995000 hasIssue "S1" @default.
- W3175995000 hasLocation W31759950001 @default.
- W3175995000 hasOpenAccess W3175995000 @default.
- W3175995000 hasPrimaryLocation W31759950001 @default.
- W3175995000 hasRelatedWork W1988642027 @default.
- W3175995000 hasRelatedWork W2011381989 @default.
- W3175995000 hasRelatedWork W2045246106 @default.
- W3175995000 hasRelatedWork W2046899302 @default.
- W3175995000 hasRelatedWork W2077063678 @default.
- W3175995000 hasRelatedWork W2350761297 @default.
- W3175995000 hasRelatedWork W2353625357 @default.
- W3175995000 hasRelatedWork W2356642702 @default.
- W3175995000 hasRelatedWork W2376162696 @default.
- W3175995000 hasRelatedWork W946343989 @default.
- W3175995000 hasVolume "23" @default.
- W3175995000 isParatext "false" @default.
- W3175995000 isRetracted "false" @default.
- W3175995000 magId "3175995000" @default.
- W3175995000 workType "article" @default.