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• W3176143910 abstract "Malfunctions in a cell's ability to degrade unnecessary chemicals or proteins may lead to one of fifty distinctive lysosomal storage disorders in humans. Lysosomal storage diseases such as Tay-Sach's, Salla, or Sialic Acid Storage diseases often lead to premature death in infants. Lysosomes are vital for degrading subcellular proteins no longer needed by the cell. Our research uses a functional homologue of lysosomes, the vacuole in S. cerevisiae yeast as a model system, which also functions in protein degradation. We study vacuoles in S. cerevisiae yeast to understand protein degradation. We investigate whether changes in vacuole size and degradative function are influenced by the progression of the cell cycle. We hypothesize that changes in vacuolar size and specific phases of the cell cycle play an important role in degradative function. To address this, we designed experiments to approach this by linking vacuole size changes to the progression of the cell cycle. We halted the cells at each cell cycle stage to track vacuolar changes as the cell cycle progresses. We did this by incubating the cells with 4 cell cycle inhibition drugs: Tributyltin (G1 phase), Hydroxyurea (S phase), Epothilone B (G2 phase), and Nocodazole (M phase). Vacuoles were fluorescently labelled with VPH1-GFP, a vacuolar surface protein. We imaged the samples using Spinning Disk Confocal Microscopy. We then analyzed the images with ImageJ and MatLab algorithms to track, compare, and characterize vacuolar change. Thus far, we have found that the vacuoles change in terms of copy number (the number of vacuolar lobes) as the cell cycle progresses. This preliminary evidence suggests that there is a correlation between changes in vacuolar size along with the progression of the cell cycle. Because of this, we hypothesize that vacuole size is influenced by the progression of the cell cycle. In addition, decreases in degradative ability are correlated with enlarged vacuolar phenotypes, hence a logical future direction would be to test whether degradative ability may also be synchronized with the progression of the cell cycle. Characterizing these changes would allow us to predict degradative efficiency based on the phase of the cell cycle. Further research could determine whether protein degradation and vacuolar morphology depend on one another. Overall, our research is significant because it will provide molecular insight on how vacuolar morphology and degradation are regulated, which may lead to new treatments to help those who suffer from lethal lysosomal storage disorders, and those who struggle with inefficiencies in degradative ability. Support or Funding Information NSF-Center for Cellular Construction Grant This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal." @default.
• W3176143910 created "2021-07-05" @default.
• W3176143910 creator A5011668134 @default.
• W3176143910 date "2018-04-01" @default.
• W3176143910 modified "2023-09-26" @default.
• W3176143910 title "Effects of the Cell Cycle on Vacuole Size in S. cerevisiae yeast" @default.
• W3176143910 doi "https://doi.org/10.1096/fasebj.2018.32.1_supplement.542.9" @default.
• W3176143910 hasPublicationYear "2018" @default.
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